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    Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State

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    Author
    Mathur, Mohit C.; Kobayashi, Tomoyoshi; Chalovich, Joseph
    Abstract
    We examined four cardiomyopathy-causing mutations of troponin I that appear to disturb function by altering the distribution of thin filament states. The R193H (mouse) troponin I mutant had greater than normal actin-activated myosin-S1 ATPase activity in both the presence and absence of calcium. The rate of ATPase activity was the same as that of the wildtype at near-saturating concentrations of the activator, N-ethylmaleimide-S1. This mutant appeared to function by stabilizing the active state of thin filaments. Mutations D191H, R146G, and R146W had lower ATPase activities in the presence of calcium, but higher activities in the absence of calcium. These effects were most pronounced with mutations at position 146. For all three mutants the rates were similar to those of the wild-type at near-saturating concentrations of N-ethylmaleimide-S1. These results, combined with previous results, show that any alteration in the normal distribution of actomyosin states is capable of producing cardiomyopathy. The results of the D191H, R146G, and R146W mutations are most readily explained if the intermediate state of regulated actin has a unique function. The intermediate state appears to have an ability to accelerate the rate of ATP hydrolysis by myosin that exceeds that of the inactive state. Originally published Biophysical Journal, Vol. 96, No. 2, March 2009
    URI
    http://hdl.handle.net/10342/3053
    Subject
     Cardiomyopathies; Troponin mutations; Intermediate actin state 
    Date
    2009-03-18
    Citation:
    APA:
    Mathur, Mohit C., & Kobayashi, Tomoyoshi, & Chalovich, Joseph. (March 2009). Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State. Biophysical Journal, 96(6), 2237- 2244. Retrieved from http://hdl.handle.net/10342/3053

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Mathur, Mohit C., and Kobayashi, Tomoyoshi, and Chalovich, Joseph. "Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State". Biophysical Journal. 96:6. (2237-2244), March 2009. September 27, 2023. http://hdl.handle.net/10342/3053.
    Chicago:
    Mathur, Mohit C. and Kobayashi, Tomoyoshi and Chalovich, Joseph, "Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State," Biophysical Journal 96, no. 6 (March 2009), http://hdl.handle.net/10342/3053 (accessed September 27, 2023).
    AMA:
    Mathur, Mohit C., Kobayashi, Tomoyoshi, Chalovich, Joseph. Some Cardiomyopathy-Causing Troponin I Mutations Stabilize a Functional Intermediate Actin State. Biophysical Journal. March 2009; 96(6): 2237-2244. http://hdl.handle.net/10342/3053. Accessed September 27, 2023.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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