• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Internal Medicine
    • View Item
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Internal Medicine
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Mechanisms controlling the acquisition of a cardiac phenotype by liver stem cells

    Thumbnail
    View/ Open
    Mechanisms control cardiac phenotype.pdf (516.4Kb)

    Show full item record
    
    Author
    Muller-Borer, Barbara J.; Cascio, Wayne E.; Esch, Gwyn L.; Kim, Hyung-Suk; Coleman, William B.; Grisham, Joe W.; Anderson, Page A. W.; Malouf, Nadia N.
    Abstract
    The mechanisms underlying stem cell acquisition of a cardiac phenotype are unresolved. We studied early events during the acquisition of a cardiac phenotype by a cloned adult liver stem cell line (WB F344) in a cardiac microenvironment. WB F344 cells express a priori the transcription factors GATA4 and SRF, connexin 43 in the cell membrane, and myoinositol 1,4,5-triphosphate receptor in the perinuclear region. Functional cell– cell communication developed between WB F344 cells and adjacent cocultured cardiomyocytes in 24 h. De novo cytoplasmic [Ca2 +]c and nuclear [Ca2+ ]nu oscillations appeared in WB F344 cells, synchronous with [Ca2+ ]i transients in adjacent cardiomyocytes. The [Ca2 +] oscillations in the WB F344 cells, but not those in the cardiomyocytes, were eliminated by a gap junction uncoupler and reappeared with its removal. By 24 h, WB F344 cells began expressing the cardiac transcription factors Nkx2.5, Tbx5, and cofactor myocardin; cardiac proteins 24 h later; and a sarcomeric pattern 4–6 days later. Myoinositol 1,4,5-triphosphate receptor inhibition suppressed WB F344 cell [Ca2 +]nu oscillations but not [Ca2+ ]c oscillations, and L-type calcium channel inhibition eliminated [Ca2 +] oscillations in cardiomyocytes and WB F344 cells. The use of these inhibitors was associated with a decrease in Nkx2.5, Tbx5, and myocardin expression in the WB F344 cells. Our findings suggest that signals from cardiomyocytes diffuse through shared channels, inducing [Ca2 +] oscillations in the WB F344 cells. We hypothesize that the WB F344 cell [Ca2 +]nu oscillations activate the expression of a cardiac specifying gene program, ushering in a cardiac phenotype. Originally published Proc Natl Acad Sci, Vol. 104, No. 10, March 2007
    URI
    http://hdl.handle.net/10342/3060
    Subject
     Calcium signal transduction; Cardiomyogenesis; Gap junction 
    Date
    2007-03-06
    Citation:
    APA:
    Muller-Borer, Barbara J., & Cascio, Wayne E., & Esch, Gwyn L., & Kim, Hyung-Suk, & Coleman, William B., & Grisham, Joe W., & Anderson, Page A. W., & Malouf, Nadia N.. (March 2007). Mechanisms controlling the acquisition of a cardiac phenotype by liver stem cells. , (. Retrieved from http://hdl.handle.net/10342/3060

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Muller-Borer, Barbara J., and Cascio, Wayne E., and Esch, Gwyn L., and Kim, Hyung-Suk, and Coleman, William B., and Grisham, Joe W., and Anderson, Page A. W., and Malouf, Nadia N.. "Mechanisms controlling the acquisition of a cardiac phenotype by liver stem cells". . . (.), March 2007. March 01, 2021. http://hdl.handle.net/10342/3060.
    Chicago:
    Muller-Borer, Barbara J. and Cascio, Wayne E. and Esch, Gwyn L. and Kim, Hyung-Suk and Coleman, William B. and Grisham, Joe W. and Anderson, Page A. W. and Malouf, Nadia N., "Mechanisms controlling the acquisition of a cardiac phenotype by liver stem cells,"  , no. (March 2007), http://hdl.handle.net/10342/3060 (accessed March 01, 2021).
    AMA:
    Muller-Borer, Barbara J., Cascio, Wayne E., Esch, Gwyn L., Kim, Hyung-Suk, Coleman, William B., Grisham, Joe W., Anderson, Page A. W., Malouf, Nadia N.. Mechanisms controlling the acquisition of a cardiac phenotype by liver stem cells. . March 2007; () . http://hdl.handle.net/10342/3060. Accessed March 01, 2021.
    Collections
    • Internal Medicine
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback