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    Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.

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    Author
    Smith, C. Jeffrey; Callihan, Donald R.
    Abstract
    The Escherichia coli rRNA operon rrnB was used as a 32P-labeled hybridization probe in Southern blots of genomic DNAs from representative strains of the saccharolytic, gram-negative, obligate anaerobes of the genus Bacteroides. Control experiments with the B. fragilis type strain ATCC 25285 established that nearly identical rRNA fragment patterns were produced when either the E. coli rrnB gene probe or homologous rRNA isolated from B. fragilis was used as the probe. In addition, it was shown that a specific 16S or 23S rrnB gene probe also could be used to produce fragment patterns suitable for analysis. Thirty-one strains from 8 of the 10 recognized Bacteroides species were then examined. The resulting autoradiographs revealed specific fragment patterns for all but one (B. ovatus) of the species tested. Restriction fragment length polymorphisms were observed for many of the strains tested, but these differences did not hinder species classification. The five B. ovatus strains examined did not form a distinct group, and their rRNA fragment patterns displayed a marked heterogeneity. The same approach was applied to a unique set of enterotoxin-producing B. fragilis strains isolted from animals and humans with diarrhea. The results demonstrated that these strains were in fact B. fragilis and that they produce rRNA fragment patterns closely related to those of the type strain ATCC 25285. This set of strains did not appear to form a separate subgroup or genotype within the B. fragilis species, and there were no distinguishable restriction fragment length polymorphisms that could be used to specifically separate enterotoxin-producing strains from nonenterotoxigenic strains. Originally published Journal of Clinical Microbiology, Vol. 30, No. 4, Apr 1992
    URI
    http://hdl.handle.net/10342/3094
    Subject
     Bacteroides fragilis; Enterotoxin production; RRNA polymorphisms 
    Date
    1992-04
    Citation:
    APA:
    Smith, C. Jeffrey, & Callihan, Donald R.. (April 1992). Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.. Journal of Clinical Microbiology, 30(4), 806- 812. Retrieved from http://hdl.handle.net/10342/3094

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Smith, C. Jeffrey, and Callihan, Donald R.. "Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.". Journal of Clinical Microbiology. 30:4. (806-812), April 1992. September 21, 2023. http://hdl.handle.net/10342/3094.
    Chicago:
    Smith, C. Jeffrey and Callihan, Donald R., "Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.," Journal of Clinical Microbiology 30, no. 4 (April 1992), http://hdl.handle.net/10342/3094 (accessed September 21, 2023).
    AMA:
    Smith, C. Jeffrey, Callihan, Donald R.. Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.. Journal of Clinical Microbiology. April 1992; 30(4): 806-812. http://hdl.handle.net/10342/3094. Accessed September 21, 2023.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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