Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.
Author
Smith, C. Jeffrey; Bennett, Tamara K.; Parker, Anita C.
Abstract
The gene, cbl, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from Bacteroides uniforinis WAL-7088. The nucleotide sequence was determined, and the cbl structural gene was found to be 891 nucleotides, with a 48% G+C composition, which is similar to that of the B. uniformis genome. The cbhi open reading frame encoded an Ambler class A ,B-lactamase polypeptide precursor of 296 amino acid residues with a predicted molecular weight of 33,450. A ,-lactamase-deficient B. uniformis mutant with increased ,-lactam susceptibility was constructed by insertional inactivation of the chromosomal gene. This mutant was complemented by plasmids bearing the cblA gene, and the resulting strains were resistant to cephaloridine and had a P-lactamase that comigrated with the parental j-lactamase on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (30,500 Da) and in isoelectric focusing gels (pl 4.6), confirming a role for this P-lactamase in resistance. Originally published Antimicrobial Agents and Chemotherapy, Vol. 38, No. 8, Aug 1994
Date
1994-08
Citation:
APA:
Smith, C. Jeffrey, & Bennett, Tamara K., & Parker, Anita C.. (August 1994).
Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase..
Antimicrobial Agents and Chemotherapy,
38(8),
1711-
1715. Retrieved from
http://hdl.handle.net/10342/3111
MLA:
Smith, C. Jeffrey, and Bennett, Tamara K., and Parker, Anita C..
"Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.". Antimicrobial Agents and Chemotherapy.
38:8. (1711-1715),
August 1994.
September 30, 2023.
http://hdl.handle.net/10342/3111.
Chicago:
Smith, C. Jeffrey and Bennett, Tamara K. and Parker, Anita C.,
"Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.," Antimicrobial Agents and Chemotherapy 38, no.
8 (August 1994),
http://hdl.handle.net/10342/3111 (accessed
September 30, 2023).
AMA:
Smith, C. Jeffrey, Bennett, Tamara K., Parker, Anita C..
Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.. Antimicrobial Agents and Chemotherapy.
August 1994;
38(8):
1711-1715.
http://hdl.handle.net/10342/3111. Accessed
September 30, 2023.
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Publisher
East Carolina University