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    Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen

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    Author
    Spence, Cheryl; Wells, W. Greg; Smith, C. Jeffrey
    Abstract
    The opportunistic pathogen Bacteroides fragilis is a commensal organism in the large intestine, where it utilizes both dietary and host-derived polysaccharides as a source of carbon and energy. In this study, a four-gene operon required for starch utilization was identified. The operon also was found to be oxygen responsive and thus was designated osu for oxygen-induced starch utilization. The first three genes in the operon were predicted to encode outer membrane proteins involved in starch binding, and a fourth gene, osuD, encoded an amylase involved in starch hydrolysis. Insertional mutation of the osuA gene ( osuA) resulted in the inability to utilize starch or glycogen and an insertional mutation into the osuD gene ( osuD) was severely impaired for growth on starch media. Transcriptional studies indicated that maltose, maltooligosaccharides, and starch were inducers of osu expression and that maltose was the strongest inducer. A transcriptional activator of osuABCD, OsuR, was identified and found to mediate maltose induction. The osuA and osuD mutants were able to grow on maltose but not starch, whereas a mutation in osuR abolished growth on both substrates, indicating that additional genes under the control of OsuR are needed for maltose utilization. The osuABCD operon also was induced by exposure to oxygen and was shown to be part of the oxidative stress response important for aerotolerance of B. fragilis. Transcriptional analyses showed that osuA was induced 20-fold by oxygen, but OsuR was not required for this activation. Analysis of osu mutants suggested that expression of the operon was important for survival during oxygen exposure but not to hydrogen peroxide stress. Originally published Journal of Bacteriology, Vol. 188, No. 13, July 2006
    URI
    http://hdl.handle.net/10342/3119
    Subject
     Starch utilization; Aerotolerance; Bacteroides fragilis 
    Date
    2006-07
    Citation:
    APA:
    Spence, Cheryl, & Wells, W. Greg, & Smith, C. Jeffrey. (July 2006). Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen. Journal of Bacteriology, (188:13), p.4663-4672. Retrieved from http://hdl.handle.net/10342/3119

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    MLA:
    Spence, Cheryl, and Wells, W. Greg, and Smith, C. Jeffrey. "Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen". Journal of Bacteriology. 188:13. (4663-4672.), July 2006. August 10, 2022. http://hdl.handle.net/10342/3119.
    Chicago:
    Spence, Cheryl and Wells, W. Greg and Smith, C. Jeffrey, "Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen," Journal of Bacteriology 188, no. 13 (July 2006), http://hdl.handle.net/10342/3119 (accessed August 10, 2022).
    AMA:
    Spence, Cheryl, Wells, W. Greg, Smith, C. Jeffrey. Characterization of the Primary Starch Utilization Operon in the Obligate Anaerobe Bacteroides fragilis: Regulation by Carbon Source and Oxygen. Journal of Bacteriology. July 2006; 188(13) 4663-4672. http://hdl.handle.net/10342/3119. Accessed August 10, 2022.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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