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    Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study

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    Author
    Wang, Hui; Mao, Shu; Chalovich, Joseph; Marriott, Gerard
    Abstract
    Cryoelectron microscopy studies have identified distinct locations of tropomyosin (Tm) within the Ca21-free, Ca21-saturated, and myosin-S1-saturated states of the thin filament. On the other hand, steady-state Förster resonance energy transfer (FRET) studies using functional, reconstituted thin filaments under physiological conditions of temperature and solvent have failed to detect any movement of Tm upon Ca21 binding. In this investigation, an optimized system for FRET and anisotropy analyses of cardiac tropomyosin (cTm) dynamics was developed that employed a single tethered donor probe within a Tm dimer. Multisite FRET and fluorescence anisotropy analyses showed that S1 binding to Ca21 thin filaments triggered a uniform displacement of cTm toward F-actin but that Ca21 binding alone did not change FRET efficiency, most likely due to thermally driven fluctuations of cTm on the thin filament that decreased the effective separation of the donor probe between the blocked and closed states. Although Ca21 binding to the thin filament did not significantly change FRET efficiency, such a change was demonstrated when the thin filament was partially saturated with S1. FRET was also used to show that stoichiometric binding of S1 to Ca21-activated thin filaments decreased the amplitude of Tm fluctuations and revealed a strong correlation between the cooperative binding of S1 to the closed state and the movement of cTm. Originally published Biophysical Journal, Vol. 94, No. 11, June 2008
    URI
    http://hdl.handle.net/10342/3133
    Subject
     Cryoelectron microscopy; Cardiac thin filaments; Tropomyosin 
    Date
    2008-06
    Citation:
    APA:
    Wang, Hui, & Mao, Shu, & Chalovich, Joseph, & Marriott, Gerard. (June 2008). Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study. Biophysical Journal, 94(11), 4358- 4369. Retrieved from http://hdl.handle.net/10342/3133

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Wang, Hui, and Mao, Shu, and Chalovich, Joseph, and Marriott, Gerard. "Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study". Biophysical Journal. 94:11. (4358-4369), June 2008. September 30, 2023. http://hdl.handle.net/10342/3133.
    Chicago:
    Wang, Hui and Mao, Shu and Chalovich, Joseph and Marriott, Gerard, "Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study," Biophysical Journal 94, no. 11 (June 2008), http://hdl.handle.net/10342/3133 (accessed September 30, 2023).
    AMA:
    Wang, Hui, Mao, Shu, Chalovich, Joseph, Marriott, Gerard. Tropomyosin Dynamics in Cardiac Thin Filaments: A Multisite Förster Resonance Energy Transfer and Anisotropy Study. Biophysical Journal. June 2008; 94(11): 4358-4369. http://hdl.handle.net/10342/3133. Accessed September 30, 2023.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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