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    WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD

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    Author
    McGrath, Jacqueline
    Abstract
    Intracellular pH plays an important role in many biological processes: receptor-mediated signal transduction, enzymatic activity, cell growth and death, ion transport and homeostasis, calcium regulation, endocytosis, chemotaxis, and cell adhesion. Normal cell pH is around 7.40 and can range from 7.35-7.45 without any adverse effects. If it ranges more than 0.1-0.2 in either direction cardiopulmonary and neurologic problems can arise and in the case of extreme variations, death can result. Determination of pH in cells is of great importance and many methods exist for sensing pH; however, fluorescence is the most useful because of its nondestructive nature, high sensitivity, and specificity. We chose to use an intrinsic fluorescent probe in which the protonation site is integrated into the main chromophore. The systems were prepared by Sonogashira coupling of halopyridines with acetylenes. Protonation of the first class of pyridyl receptors synthesized occurred at a pH of 4 and was accompanied by a decrease in fluorescence. Protonation of the second class of pyridyl acceptor synthesized occurs near the pH of 5.5 and is accompanied by an increase in fluorescence intensity. Though water-soluble, model membrane transport studies show that the probes have a high affinity for non polar environments.  
    URI
    http://hdl.handle.net/10342/3180
    Subject
     Chemistry, Organic; Organic chemistry 
    Date
    2010
    Citation:
    APA:
    McGrath, Jacqueline. (January 2010). WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/3180.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    McGrath, Jacqueline. WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD. Master's Thesis. East Carolina University, January 2010. The Scholarship. http://hdl.handle.net/10342/3180. December 04, 2023.
    Chicago:
    McGrath, Jacqueline, “WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD” (Master's Thesis., East Carolina University, January 2010).
    AMA:
    McGrath, Jacqueline. WATER-SOLUBLE FLUORESCENT PH SENSORS BASED ON A DIARYLACETYLENE SCAFFOLD [Master's Thesis]. Greenville, NC: East Carolina University; January 2010.
    Collections
    • Chemistry
    • Master's Theses
    Publisher
    East Carolina University

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