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    Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle

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    Author
    Bellaire, Bryan H.; Elzer, Philip H.; Hagius, Sue; Walker, Joel; Baldwin, Cynthia L.; Roop, R. Martin II
    Abstract
    Brucella abortus reportedly produces the monocatechol siderophore 2,3-dihydroxybenzoic acid (2,3-DHBA) in response to iron limitation. Nucleotide sequence analysis of the cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined Escherichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsible for synthesis of this siderophore. Reverse transcriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene. Homologs of the E. coli entD and Vibrio cholerae vibH genes were also detected in the flanking regions immediately adjacent to the B. abortus dhbCEBA operon, suggesting that B. abortus has the genetic capacity to produce a more complex 2,3-DHBA-based siderophore. Slot blot hybridization experiments and primer extension analysis showed that transcription of the B. abortus dhbCEBA operon originates from two iron-regulated promoters located upstream of dhbC. Consistent with their iron-dependent regulation, both of the dhbCEBA promoter sequences contain typical consensus Fur-binding motifs. Although previously published studies have shown that 2,3-DHBA production is not required for the establishment and maintenance of chronic spleen infection by B. abortus in mice, experimental infection of pregnant cattle with the B. abortus dhbC mutant BHB1 clearly showed that production of this siderophore is essential for wild-type virulence in the natural ruminant host. Originally published Infection and Immunity, Vol. 71, No. 4, Apr 2003
    URI
    http://hdl.handle.net/10342/3203
    Subject
     2,3-dihydroxybenzoic acid; Brucella abortus; Iron limitation 
    Date
    2003-04
    Citation:
    APA:
    Bellaire, Bryan H., & Elzer, Philip H., & Hagius, Sue, & Walker, Joel, & Baldwin, Cynthia L., & Roop, R. Martin II. (April 2003). Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle. Infection and Immunity, (71:4), p.1794-1803. Retrieved from http://hdl.handle.net/10342/3203

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Bellaire, Bryan H., and Elzer, Philip H., and Hagius, Sue, and Walker, Joel, and Baldwin, Cynthia L., and Roop, R. Martin II. "Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle". Infection and Immunity. 71:4. (1794-1803.), April 2003. August 10, 2022. http://hdl.handle.net/10342/3203.
    Chicago:
    Bellaire, Bryan H. and Elzer, Philip H. and Hagius, Sue and Walker, Joel and Baldwin, Cynthia L. and Roop, R. Martin II, "Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle," Infection and Immunity 71, no. 4 (April 2003), http://hdl.handle.net/10342/3203 (accessed August 10, 2022).
    AMA:
    Bellaire, Bryan H., Elzer, Philip H., Hagius, Sue, Walker, Joel, Baldwin, Cynthia L., Roop, R. Martin II. Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle. Infection and Immunity. April 2003; 71(4) 1794-1803. http://hdl.handle.net/10342/3203. Accessed August 10, 2022.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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