Transplantation of GABAergic Neurons but not Astrocytes Induces Recovery of Sensorimotor Function in the Traumatically Injured Brain
Author
Becerra, G. D.; Tatko, L. M.; Pak, Elena S.; Murashov, Alexander K.; Hoane, M. R.
Abstract
Embryonic stem (ES) cells have been investigated in many animal models of injury and disease.
However, few studies have examined the ability of pre-differentiated ES cells to improve functional
outcome following traumatic brain injury (TBI). The purpose of the present study was to compare
the effect of murine ES cells that were pre-differentiated into GABAergic neurons or astrocytes on
functional recovery following TBI. Neural and astrocyte induction was achieved by co-culturing ES
cells on a bone marrow stromal fibroblast (M2-10B4) feeder layer and incubating them with various
mitogenic factors. Rats were initially prepared with a unilateral controlled cortical contusion injury
of the sensorimotor cortex or sham procedure. Rats were transplanted 7 days following injury with
∼100K GABAergic neurons, astrocytes, fibroblasts, or media. Animals were assessed on a battery
of sensorimotor tasks following transplantation. The stromal fibroblast cells (M2-10B4), as a control
cell line, did not differ significantly from media infusions. Transplantation of GABAergic neurons
facilitated complete and total recovery on the vibrissae-forelimb placing test as opposed to all other
groups, which failed to show any recovery. It was also found that GABAergic neurons reduced the
magnitude of the initial impairment on the limb use test. Histological analysis revealed infiltration
of host brain with transplanted neurons and astrocytes. The results of the present study suggest that
transplantation of pre-differentiated GABAergic neurons significantly induces recovery of
sensorimotor function; whereas, astrocytes do not. Originally published Behav Brain Res, Vol. 179, No. 1, Apr 2007
Date
2007-04-16
Citation:
APA:
Becerra, G. D., & Tatko, L. M., & Pak, Elena S., & Murashov, Alexander K., & Hoane, M. R.. (April 2007).
Transplantation of GABAergic Neurons but not Astrocytes Induces Recovery of Sensorimotor Function in the Traumatically Injured Brain.
Behavioural Brain Research,
179(1),
118-
125. Retrieved from
http://hdl.handle.net/10342/3209
MLA:
Becerra, G. D., and Tatko, L. M., and Pak, Elena S., and Murashov, Alexander K., and Hoane, M. R..
"Transplantation of GABAergic Neurons but not Astrocytes Induces Recovery of Sensorimotor Function in the Traumatically Injured Brain". Behavioural Brain Research.
179:1. (118-125),
April 2007.
September 29, 2023.
http://hdl.handle.net/10342/3209.
Chicago:
Becerra, G. D. and Tatko, L. M. and Pak, Elena S. and Murashov, Alexander K. and Hoane, M. R.,
"Transplantation of GABAergic Neurons but not Astrocytes Induces Recovery of Sensorimotor Function in the Traumatically Injured Brain," Behavioural Brain Research 179, no.
1 (April 2007),
http://hdl.handle.net/10342/3209 (accessed
September 29, 2023).
AMA:
Becerra, G. D., Tatko, L. M., Pak, Elena S., Murashov, Alexander K., Hoane, M. R..
Transplantation of GABAergic Neurons but not Astrocytes Induces Recovery of Sensorimotor Function in the Traumatically Injured Brain. Behavioural Brain Research.
April 2007;
179(1):
118-125.
http://hdl.handle.net/10342/3209. Accessed
September 29, 2023.
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Publisher
East Carolina University