The Interaction of Caldesmon with the COOH Terminus of Actin
Crosbie, Rachelle; Adams, Susan; Chalovich, Joseph; Reisler, Emil
Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon both in the presence and absence of tropomyosin. Furthermore, as revealed by fluorescence measurements, caldesmon increases the exposure of the COOH-terminal region of actin to the solvent. This effect of caldesmon, like its inhibitory effect on actomyosin ATPase activity, is enhanced in the presence of tropomyosin. Proteolytic removal of the last three COOH-terminal residues of actin, containing the modified cysteine residue, restores the normal binding between caldesmon and actin. These results establish a correlation between the binding of caldesmon to actin and the conformation of the COOH-terminal region of actin and suggest an indirect rather than direct interaction between caldesmon and this part of actin. Originally published Journal of Biological Chemistry, Vol. 266, No. 30, Oct 1991
Crosbie, Rachelle, & Adams, Susan, & Chalovich, Joseph, & Reisler, Emil. (October 1991). The Interaction of Caldesmon with the COOH Terminus of Actin. Journal of Biological Chemistry, 266(30), 20001- 20006. Retrieved from http://hdl.handle.net/10342/3251
Crosbie, Rachelle, and Adams, Susan, and Chalovich, Joseph, and Reisler, Emil. "The Interaction of Caldesmon with the COOH Terminus of Actin". Journal of Biological Chemistry. 266:30. (20001-20006), October 1991. September 22, 2023. http://hdl.handle.net/10342/3251.
Crosbie, Rachelle and Adams, Susan and Chalovich, Joseph and Reisler, Emil, "The Interaction of Caldesmon with the COOH Terminus of Actin," Journal of Biological Chemistry 266, no. 30 (October 1991), http://hdl.handle.net/10342/3251 (accessed September 22, 2023).
Crosbie, Rachelle, Adams, Susan, Chalovich, Joseph, Reisler, Emil. The Interaction of Caldesmon with the COOH Terminus of Actin. Journal of Biological Chemistry. October 1991; 266(30): 20001-20006. http://hdl.handle.net/10342/3251. Accessed September 22, 2023.
East Carolina University