The effect of fasting on the activation in vivo of the insulin receptor kinase.
Contreras, Ivan; Dohm, G. Lynis; Abdallah, Silvia; Wells, James A.; Mooney, Nitin; Rovira, Adela; Caro, Jose F.
Fasting causes insulin resistance in liver and fat, and increases insulin sensitivity in muscle. We studied the response in vitro and in vivo to insulin of the insulin receptor tyrosine kinase in muscle and liver from 72 h fasted and control rats. Insulin was injected intraperitoneally together with glucose, and blood and tissue samples were obtained 0, 5, 15 and 30 min later. Basal serum glucose and insulin levels were significantly higher in control than in fasting rats. Serum glucose rose to 300mg/dl at 5 min and then progressively declined without hypoglycaemia. Receptors were prepared from whole tissueby wheat germ lectinafinity chromatography. 125I-insulin binding to purified receptors was increased by fasting in both muscle (18%) and liver (50%). In untreated fasting and control animals, muscle and liver insulin receptor tyrosine kinase activity was stimulated to similar levels by insulin added in vitro. With only insulin treatment in vivo, muscle receptor tyrosine kinase behaved similarly in fasting and control animals with maximal activation at 15 min post injection. In liver, insulin in vivo stimulated receptor tyrosine kinase activity maximally at 5 min post injection in both fasting and control, but in fasting animals the treatment in vivo caused a significantly larger and more prolonged activation of the enzymic activity, possibly due to a decrease in the rate of dephosphorylation and deactivation of the beta subunits. Originally published Biochemical Journal, Vol. 265, No. 3, Feb 1990
Contreras, Ivan, & Dohm, G. Lynis, & Abdallah, Silvia, & Wells, James A., & Mooney, Nitin, & Rovira, Adela, & Caro, Jose F.. (February 1990). The effect of fasting on the activation in vivo of the insulin receptor kinase.. Biochemical Journal, (265:3), p.887-890. Retrieved from http://hdl.handle.net/10342/3253
Contreras, Ivan, and Dohm, G. Lynis, and Abdallah, Silvia, and Wells, James A., and Mooney, Nitin, and Rovira, Adela, and Caro, Jose F.. "The effect of fasting on the activation in vivo of the insulin receptor kinase.". Biochemical Journal. 265:3. (887-890.), February 1990. June 16, 2021. http://hdl.handle.net/10342/3253.
Contreras, Ivan and Dohm, G. Lynis and Abdallah, Silvia and Wells, James A. and Mooney, Nitin and Rovira, Adela and Caro, Jose F., "The effect of fasting on the activation in vivo of the insulin receptor kinase.," Biochemical Journal 265, no. 3 (February 1990), http://hdl.handle.net/10342/3253 (accessed June 16, 2021).
Contreras, Ivan, Dohm, G. Lynis, Abdallah, Silvia, Wells, James A., Mooney, Nitin, Rovira, Adela, Caro, Jose F.. The effect of fasting on the activation in vivo of the insulin receptor kinase.. Biochemical Journal. February 1990; 265(3) 887-890. http://hdl.handle.net/10342/3253. Accessed June 16, 2021.
East Carolina University