Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids
Author
Ferguson, Gail P.; Datta, Anup; Baumgartner, John; Roop, R. Martin II; Carlson, Russ W.; Walker, Graham C.
Abstract
Sinorhizobium meliloti, a legume symbiont, and Brucella abortus, a phylogenetically related mammalian pathogen, both require the bacterial-encoded BacA protein to establish chronic intracellular infections in their respective hosts. We found that the bacterial BacA proteins share sequence similarity with a family of eukaryotic peroxisomal-membrane proteins, including the human adrenoleukodystrophy protein, required for the efficient transport of verylong- chain fatty acids out of the cytoplasm. This insight, along with the increased sensitivity of BacA-deficient mutants to detergents and cell envelope-disrupting agents, led us to discover that BacA affects the very-long-chain fatty acid (27-OHC28:0 and 29-OHC30:0) content of both Sinorhizobium and Brucella lipid A. We discuss models for how BacA function affects the lipid-A fatty-acid content and why this activity could be important for the establishment of chronic intracellular infections. Originally published Proceedings of the National Academy of Sciences, Vol. 101, No. 14, Apr 2004
Date
2004-04-06
Citation:
APA:
Ferguson, Gail P., & Datta, Anup, & Baumgartner, John, & Roop, R. Martin II, & Carlson, Russ W., & Walker, Graham C.. (April 2004).
Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids.
Proceedings of the National Academy of Sciences,
101(14),
5012-
5017. Retrieved from
http://hdl.handle.net/10342/3258
MLA:
Ferguson, Gail P., and Datta, Anup, and Baumgartner, John, and Roop, R. Martin II, and Carlson, Russ W., and Walker, Graham C..
"Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids". Proceedings of the National Academy of Sciences.
101:14. (5012-5017),
April 2004.
September 23, 2023.
http://hdl.handle.net/10342/3258.
Chicago:
Ferguson, Gail P. and Datta, Anup and Baumgartner, John and Roop, R. Martin II and Carlson, Russ W. and Walker, Graham C.,
"Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids," Proceedings of the National Academy of Sciences 101, no.
14 (April 2004),
http://hdl.handle.net/10342/3258 (accessed
September 23, 2023).
AMA:
Ferguson, Gail P., Datta, Anup, Baumgartner, John, Roop, R. Martin II, Carlson, Russ W., Walker, Graham C..
Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids. Proceedings of the National Academy of Sciences.
April 2004;
101(14):
5012-5017.
http://hdl.handle.net/10342/3258. Accessed
September 23, 2023.
Collections
Publisher
East Carolina University