An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.
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Date
1988-08
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Authors
Dohm, G. Lynis
Tapscott, Edward B.
Pories, Walter J.
Dabbs, David J.
Flickinger, Edward G.
Meelheim, Diane
Fushiki, Tohru
Atkinson, Samuel M.
Elton, Charles W.
Caro, Jose F.
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Publisher
East Carolina University
Abstract
We have developed an in vitro muscle preparation suitable for metabolic studies with human muscle tissue and have investigated the effects of obesity and non-insulin-dependent diabetes mellitus (NIDDM) on glucose transport. Transport of 3-0- methylglucose and 2-deoxyglucose was stimulated approximately twofold by insulin in muscle from normal nonobese subjects and stimulation occurred in the normal physiological range of insulin concentrations. In contrast to insulin stimulation of 3-0-methylglucose and 2-deoxyglucose transport in muscle from normal, nonobese subjects, tissue from morbidly obese subjects, with or without NIDDM, were not responsive to insulin. Maximal 3-0-methylglucose transport was lower in muscle of obese than nonobese subjects.
Morbidly obese patients, with or without NIDDM, have a severe state of insulin resistance in glucose transport. The novel in vitro human skeletal muscle preparation herein described should be useful in investigating the mechanism of this insulin resistance. Originally published Journal of Clinical investigation, Vol. 82, No. 2, Aug 1988
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Citation
Journal of Clinical Investigation; 82:2 p. 486-494
DOI
10.1172/JCI113622