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    Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins

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    Author
    Dyson, Ossie F.; Oxendine, Telisha L.; Hamden, Khalief E.; Ford, Patrick W.; Akula, Shaw M.
    Abstract
    Kaposi’s sarcoma-associated herpesvirus (KSHV) has two modes replication: latent and lytic replication. Reactivation from latency is dictated, in part, by the cell cycle. Herein, we have attempted to delineate the importance of cell cycle in KSHV pathogenesis by exploring the expression pattern of cell surface receptors during different phases of the cell cycle. αV integrin expression is augmented during S phase in fibroblasts, epithelial, and KSHV infected cells. Using a Matrigel system, we pioneer the concept that KSHV infected primary effusion lymphoma (PEL) cells can attach to extracellular matrix proteins. This attachment is mediated primarily via αV integrins or virally encoded gB, and occurs preferentially in cells from S phase or cells from S phase actively supporting a lytic infection, respectively. Such an ability of infected B cells to attach to endothelial cells may also aid in the dissemination of infection. The keystone of this work is that for the first time, we describe the ability of KSHV infected B cells to preferentially use cellular (αV) or viral (gB) receptors to specifically bind cells, depending upon the stage of the cell cycle and infection. Originally published Cellular Microbiology, Vol. 10, No. 7, July 2008
    URI
    http://hdl.handle.net/10342/3264
    Subject
     KSHV; HHV-8; Cellular attachment; GB; Alpha-V; Integrins; Matrigel; Reactivation 
    Date
    2008-07
    Citation:
    APA:
    Dyson, Ossie F., & Oxendine, Telisha L., & Hamden, Khalief E., & Ford, Patrick W., & Akula, Shaw M.. (July 2008). Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins. Cellular Microbiology, 10(7), 1546- 1558. Retrieved from http://hdl.handle.net/10342/3264

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Dyson, Ossie F., and Oxendine, Telisha L., and Hamden, Khalief E., and Ford, Patrick W., and Akula, Shaw M.. "Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins". Cellular Microbiology. 10:7. (1546-1558), July 2008. October 01, 2023. http://hdl.handle.net/10342/3264.
    Chicago:
    Dyson, Ossie F. and Oxendine, Telisha L. and Hamden, Khalief E. and Ford, Patrick W. and Akula, Shaw M., "Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins," Cellular Microbiology 10, no. 7 (July 2008), http://hdl.handle.net/10342/3264 (accessed October 01, 2023).
    AMA:
    Dyson, Ossie F., Oxendine, Telisha L., Hamden, Khalief E., Ford, Patrick W., Akula, Shaw M.. Differential regulation of the attachment of KSHV infected human B cells to ECM by KSHV encoded gB and cellular alpha-V integrins. Cellular Microbiology. July 2008; 10(7): 1546-1558. http://hdl.handle.net/10342/3264. Accessed October 01, 2023.
    Collections
    • Microbiology and Immunology
    Publisher
    East Carolina University

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