Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation
Author
Dyson, Ossie F.; Ford, Patrick W.; Chen, De; Li, Yong-Qing; Akula, Shaw M.
Abstract
Kaposi's sarcoma-associated herpesvirus (KSHV) has both latent and lytic phases of replication. The molecular switch that triggers a reactivation is still unclear. Cells from S phase of cell cycle provide apt conditions for an active reactivation. In order to specifically delineate the Raman spectra of cells supporting KSHV reactivation, we followed a novel approach where cells were sorted based on the state of infection (latent Vs lytic) by a flow cytometer and then analyzed by the Raman tweezers. The Raman bands at 785, 813, 830, 1095, and 1128 cm-1 are specifically altered in cells supporting KSHV reactivation. These 5 peaks make up the Raman fingerprint of cells supporting KSHV reactivation. The physiological relevance of the changes in these peaks with respect to KSHV reactivation is discussed in the following report. Originally published Journal of Cellular and Molecular Medicine, Vol. 13, No. 8b, Aug 2009
Subject
Date
2009-08
Citation:
APA:
Dyson, Ossie F., & Ford, Patrick W., & Chen, De, & Li, Yong-Qing, & Akula, Shaw M.. (August 2009).
Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation.
Journal of Cellular and Molecular Medicine,
13(8b),
1920-
1932. Retrieved from
http://hdl.handle.net/10342/3266
MLA:
Dyson, Ossie F., and Ford, Patrick W., and Chen, De, and Li, Yong-Qing, and Akula, Shaw M..
"Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation". Journal of Cellular and Molecular Medicine.
13:8b. (1920-1932),
August 2009.
September 30, 2023.
http://hdl.handle.net/10342/3266.
Chicago:
Dyson, Ossie F. and Ford, Patrick W. and Chen, De and Li, Yong-Qing and Akula, Shaw M.,
"Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation," Journal of Cellular and Molecular Medicine 13, no.
8b (August 2009),
http://hdl.handle.net/10342/3266 (accessed
September 30, 2023).
AMA:
Dyson, Ossie F., Ford, Patrick W., Chen, De, Li, Yong-Qing, Akula, Shaw M..
Raman tweezers provide the fingerprint of cells supporting the late stages of KSHV reactivation. Journal of Cellular and Molecular Medicine.
August 2009;
13(8b):
1920-1932.
http://hdl.handle.net/10342/3266. Accessed
September 30, 2023.
Collections
Publisher
East Carolina University