Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
Author
Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing
Abstract
Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many environmental stresses and in spore stability, and Ca-DPA levels in spore populations can vary with spore species/strains, as well as with sporulation conditions. We have measured levels of Ca-DPA in large numbers of individual spores in populations of a variety of Bacillus species and strains by using microfluidic Raman tweezers, in which a single spore is trapped in a focused laser beam and its Ca-DPA is quantitated from the intensity of the Ca-DPA-specific band at 1,017 cm!1 in Raman spectroscopy. Conclusions from these measurements include the following: (i) Ca-DPA concentrations in the spore core are >800 mM, well above Ca-DPA solubility; (ii) SpoVA proteins may be involved in Ca-DPA uptake in sporulation; and (iii) Ca-DPA levels differ significantly among individual spores in a population, but much of this variation could be due to variations in the sizes of individual spores. Originally published Journal of Bacteriology, Vol. 189, No. 13, July 2007
Subject
Date
2007-07
Citation:
APA:
Huang, Shu-shi, & Chen, De, & Pelczar, Patricia L., & Vepachedu, Venkata Ramana, & Setlow, Peter, & Li, Yong-Qing. (July 2007).
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers.
Journal of Bacteriology,
189(13),
4681-
4687. Retrieved from
http://hdl.handle.net/10342/3281
MLA:
Huang, Shu-shi, and Chen, De, and Pelczar, Patricia L., and Vepachedu, Venkata Ramana, and Setlow, Peter, and Li, Yong-Qing.
"Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers". Journal of Bacteriology.
189:13. (4681-4687),
July 2007.
September 22, 2023.
http://hdl.handle.net/10342/3281.
Chicago:
Huang, Shu-shi and Chen, De and Pelczar, Patricia L. and Vepachedu, Venkata Ramana and Setlow, Peter and Li, Yong-Qing,
"Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers," Journal of Bacteriology 189, no.
13 (July 2007),
http://hdl.handle.net/10342/3281 (accessed
September 22, 2023).
AMA:
Huang, Shu-shi, Chen, De, Pelczar, Patricia L., Vepachedu, Venkata Ramana, Setlow, Peter, Li, Yong-Qing.
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers. Journal of Bacteriology.
July 2007;
189(13):
4681-4687.
http://hdl.handle.net/10342/3281. Accessed
September 22, 2023.
Collections
Publisher
East Carolina University