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    Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers

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    Author
    Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing
    Abstract
    Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many environmental stresses and in spore stability, and Ca-DPA levels in spore populations can vary with spore species/strains, as well as with sporulation conditions. We have measured levels of Ca-DPA in large numbers of individual spores in populations of a variety of Bacillus species and strains by using microfluidic Raman tweezers, in which a single spore is trapped in a focused laser beam and its Ca-DPA is quantitated from the intensity of the Ca-DPA-specific band at 1,017 cm!1 in Raman spectroscopy. Conclusions from these measurements include the following: (i) Ca-DPA concentrations in the spore core are >800 mM, well above Ca-DPA solubility; (ii) SpoVA proteins may be involved in Ca-DPA uptake in sporulation; and (iii) Ca-DPA levels differ significantly among individual spores in a population, but much of this variation could be due to variations in the sizes of individual spores. Originally published Journal of Bacteriology, Vol. 189, No. 13, July 2007
    URI
    http://hdl.handle.net/10342/3281
    Subject
     Bacillus spores; Ca-DPA; Raman spectroscopy 
    Date
    2007-07
    Citation:
    APA:
    Huang, Shu-shi, & Chen, De, & Pelczar, Patricia L., & Vepachedu, Venkata Ramana, & Setlow, Peter, & Li, Yong-Qing. (July 2007). Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers. , (. Retrieved from http://hdl.handle.net/10342/3281

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Huang, Shu-shi, and Chen, De, and Pelczar, Patricia L., and Vepachedu, Venkata Ramana, and Setlow, Peter, and Li, Yong-Qing. "Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers". . . (.), July 2007. April 21, 2021. http://hdl.handle.net/10342/3281.
    Chicago:
    Huang, Shu-shi and Chen, De and Pelczar, Patricia L. and Vepachedu, Venkata Ramana and Setlow, Peter and Li, Yong-Qing, "Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers,"  , no. (July 2007), http://hdl.handle.net/10342/3281 (accessed April 21, 2021).
    AMA:
    Huang, Shu-shi, Chen, De, Pelczar, Patricia L., Vepachedu, Venkata Ramana, Setlow, Peter, Li, Yong-Qing. Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers. . July 2007; () . http://hdl.handle.net/10342/3281. Accessed April 21, 2021.
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