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    Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications

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    Author
    Gafurov, Boris; Chen, Yi-Der; Chalovich, Joseph
    Abstract
    Skeletal and cardiac muscle contraction are inhibited by the actin-associated complex of tropomyosin-troponin. Binding of Ca21 to troponin or binding of ATP-free myosin to actin reverses this inhibition. Ca21 and ATP-free myosin stabilize different tropomyosin-actin structural arrangements. The position of tropomyosin on actin affects the binding of ATP-free myosin to actin but does not greatly affect myosin-ATP binding. Ca21 and ATP-free myosin alter both the affinity of ATP-free myosin for actin and the kinetics of that binding. A parallel pathway model of regulation simulated the effects of Ca21 and ATP-free myosin binding on both equilibrium binding of myosin-nucleotide complexes to actin and the general features of ATPase activity. That model was recently shown to simulate the kinetics of myosin-S1 binding but the analysis was limited to a single condition because of the limited data available. We have now measured equilibrium binding and binding kinetics of myosin-S1-ADP to actin at a series of ionic strengths and free Ca21 concentrations. The parallel pathway model of regulation is consistent with those data. In that model the interaction between adjacent regulatory complexes fully saturated with Ca21 was destabilized and the inactive state of actin was stabilized at high ionic strength. These changes explain the previously observed change in binding kinetics with increasing ionic strength. Originally published Biophysical Journal, Vol. 87, No. 3, Sep 2004
    URI
    http://hdl.handle.net/10342/3284
    Subject
     Myosin-S1-ADP; Actin binding; Parallel pathway regulation 
    Date
    2004-09
    Citation:
    APA:
    Gafurov, Boris, & Chen, Yi-Der, & Chalovich, Joseph. (September 2004). Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications. Biophysical Journal, 87(3), 1825- 1835. Retrieved from http://hdl.handle.net/10342/3284

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Gafurov, Boris, and Chen, Yi-Der, and Chalovich, Joseph. "Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications". Biophysical Journal. 87:3. (1825-1835), September 2004. September 21, 2023. http://hdl.handle.net/10342/3284.
    Chicago:
    Gafurov, Boris and Chen, Yi-Der and Chalovich, Joseph, "Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications," Biophysical Journal 87, no. 3 (September 2004), http://hdl.handle.net/10342/3284 (accessed September 21, 2023).
    AMA:
    Gafurov, Boris, Chen, Yi-Der, Chalovich, Joseph. Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications. Biophysical Journal. September 2004; 87(3): 1825-1835. http://hdl.handle.net/10342/3284. Accessed September 21, 2023.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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