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    The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin

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    Author
    Gafurov, Boris; Fredricksen, Scott; Cai, Anmei; Brenner, Bernhard; Chase, P. Bryant; Chalovich, Joseph M.
    Abstract
    The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca2+ concentrations. Ca2+ stimulates ATP activity, and at subsaturating actin concentrations, the binding of NEM-modified S1 to actin– tropomyosin–troponin increases the rate of ATP hydrolysis even further. We show here that the ∆14 mutation of troponin T, associated with familial hypertrophic cardiomyopathy, results in an increase in ATPase rate like that seen with wild-type troponin in the presence of NEM-S1. The enhanced ATPase activity was not due to a decreased incorporation of mutant troponin T with troponin I and troponin C to form an active troponin complex. The activating effect was more prominent with a hybrid troponin (skeletal TnI, TnC, and cardiac TnT) than with all cardiac troponin. Thus it appears that changes in the troponin–troponin contacts that result from mutations or from forming hybrids stabilize a more active state of regulated actin. An analysis of the effect of the ∆14 mutation on the equilibrium binding of S1-ADP to actin was consistent with stabilization of an active state of actin. This change in activation may be important in the development of cardiac disease. Originally published Biochemistry, Vol. 43, No. 48, Dec 2004
    URI
    http://hdl.handle.net/10342/3287
    Subject
     S1-ADP; Tropomyosin; Troponin; Actin 
    Date
    2004-12-07
    Citation:
    APA:
    Gafurov, Boris, & Fredricksen, Scott, & Cai, Anmei, & Brenner, Bernhard, & Chase, P. Bryant, & Chalovich, Joseph M.. (December 2004). The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin. Biochemistry, (43:48), p.15276-15285. Retrieved from http://hdl.handle.net/10342/3287

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Gafurov, Boris, and Fredricksen, Scott, and Cai, Anmei, and Brenner, Bernhard, and Chase, P. Bryant, and Chalovich, Joseph M.. "The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin". Biochemistry. 43:48. (15276-15285.), December 2004. March 07, 2021. http://hdl.handle.net/10342/3287.
    Chicago:
    Gafurov, Boris and Fredricksen, Scott and Cai, Anmei and Brenner, Bernhard and Chase, P. Bryant and Chalovich, Joseph M., "The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin," Biochemistry 43, no. 48 (December 2004), http://hdl.handle.net/10342/3287 (accessed March 07, 2021).
    AMA:
    Gafurov, Boris, Fredricksen, Scott, Cai, Anmei, Brenner, Bernhard, Chase, P. Bryant, Chalovich, Joseph M.. The delta-14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin. Biochemistry. December 2004; 43(48) 15276-15285. http://hdl.handle.net/10342/3287. Accessed March 07, 2021.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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