Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa
Author
Gallagher, Larry A.; McKnight, Susan L.; Kuznetsova, Marina S.; Pesci, Everett C.; Manoil, Colin
Abstract
A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in the primary phenazine biosynthetic pathway and those with more pleiotropic defects. The largest set of pleiotropic mutations blocked the production of the extracellular Pseudomonas quinolone signal (PQS), a molecule required for the synthesis of secondary metabolites and extracellular enzymes. Most of these pqs mutations affected genes which appear to encode PQS biosynthetic functions, although a transcriptional regulator and an apparent response effector were also represented. Two of the genes required for PQS synthesis (phnA and phnB) had previously been assumed to encode phenazine biosynthetic functions. The transcription of one of the genes required for PQS synthesis (PA2587/pqsH) was regulated by the LasI/R quorum-sensing system, thereby linking quorum sensing and PQS regulation. Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice. Originally published Journal of Bacteriology, Vol. 184, No. 23, Dec 2002
Date
2002-12
Citation:
APA:
Gallagher, Larry A., & McKnight, Susan L., & Kuznetsova, Marina S., & Pesci, Everett C., & Manoil, Colin. (December 2002).
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa.
Journal of Bacteriology,
184(23),
6472-
6480. Retrieved from
http://hdl.handle.net/10342/3288
MLA:
Gallagher, Larry A., and McKnight, Susan L., and Kuznetsova, Marina S., and Pesci, Everett C., and Manoil, Colin.
"Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa". Journal of Bacteriology.
184:23. (6472-6480),
December 2002.
April 19, 2024.
http://hdl.handle.net/10342/3288.
Chicago:
Gallagher, Larry A. and McKnight, Susan L. and Kuznetsova, Marina S. and Pesci, Everett C. and Manoil, Colin,
"Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa," Journal of Bacteriology 184, no.
23 (December 2002),
http://hdl.handle.net/10342/3288 (accessed
April 19, 2024).
AMA:
Gallagher, Larry A., McKnight, Susan L., Kuznetsova, Marina S., Pesci, Everett C., Manoil, Colin.
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa. Journal of Bacteriology.
December 2002;
184(23):
6472-6480.
http://hdl.handle.net/10342/3288. Accessed
April 19, 2024.
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Publisher
East Carolina University