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    Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.

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    Author
    Jain, Renu G.; Andrews, Lucy G.; McGowan, Kevin M.; Pekala, Phillip H.; Keene, Jack D.
    Abstract
    3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member of the ELAV-like family of proteins associated with the induction and maintenance of differentiation in various species. ELAV proteins are known to bind in vitro to short stretches of uridylates in the 3' untranslated regions (3'UTRs) of unstable mRNAs encoding growth-regulatory proteins involved in transcription and signal transduction. GLUT1 mRNA also contains a large 3'UTR with a U-rich region that binds specifically to Hel-N1 in vitro. Analysis of the altered GLUT1 expression at the translational and posttranscriptional levels suggested a mechanism involving both mRNA stabilization and accelerated formation of translation initiation complexes. These findings are consistent with the hypothesis that the Hel-N1 family of proteins modulate gene expression at the level of mRNA in the cytoplasm. Originally published Molecular and Cellular Biology, Vol. 17, No. 2, Feb 1997
    URI
    http://hdl.handle.net/10342/3304
    Subject
     RNA binding protein; Glucose transporter; Gene expression 
    Date
    1997-02
    Citation:
    APA:
    Jain, Renu G., & Andrews, Lucy G., & McGowan, Kevin M., & Pekala, Phillip H., & Keene, Jack D.. (February 1997). Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.. Molecular and Cellular Biology, (17:2), p.954-962. Retrieved from http://hdl.handle.net/10342/3304

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Jain, Renu G., and Andrews, Lucy G., and McGowan, Kevin M., and Pekala, Phillip H., and Keene, Jack D.. "Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.". Molecular and Cellular Biology. 17:2. (954-962.), February 1997. August 10, 2022. http://hdl.handle.net/10342/3304.
    Chicago:
    Jain, Renu G. and Andrews, Lucy G. and McGowan, Kevin M. and Pekala, Phillip H. and Keene, Jack D., "Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.," Molecular and Cellular Biology 17, no. 2 (February 1997), http://hdl.handle.net/10342/3304 (accessed August 10, 2022).
    AMA:
    Jain, Renu G., Andrews, Lucy G., McGowan, Kevin M., Pekala, Phillip H., Keene, Jack D.. Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.. Molecular and Cellular Biology. February 1997; 17(2) 954-962. http://hdl.handle.net/10342/3304. Accessed August 10, 2022.
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    • Biochemistry and Molecular Biology
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    East Carolina University

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