Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.
Author
Kang, Ho Young; Brickman, Timothy J.; Beaumont, Fiona C.; Armstrong, Sandra K.
Abstract
Bordetella bronchiseptica mutants BRM1, BRM6, and BRM9 fail to produce the native dihydroxamate siderophore alcaligin. A 4.5-kb BamHI-SmaI Bordetella pertussis genomic DNA fragment carried multiple genes required to restore alcaligin production to these siderophore-deficient mutants. Phenotypic complementation analysis using subclones of the 4.5-kb genomic region demonstrated that the closely linked BRM1 and BRM9 mutations were genetically separable from the BRM6 mutation, and both insertions exerted strong polar effects on expression of the downstream gene defined by the BRM6 mutation, suggesting a polycistronic transcriptional organization of these alcaligin biosynthesis genes. Subcloning and complementation experiments localized the putative Bordetella promoter to a 0.7-kb BamHI-SphI subregion of the cloned genomic DNA fragment. Nucleotide sequencing, phenotypic analysis of mutants, and protein expression by the 4.5-kb DNA fragment in Escherichia coli suggested the presence of three alcaligin system genes, namely, alcA, alcB, and alcC. The deduced protein products of alcA, alcB, and alcC have significant primary amino acid sequence similarities with known microbial siderophore biosynthesis enzymes. Primer extension analysis mapped the transcriptional start site of the putative alcaligin biosynthesis operon containing alcABC to a promoter region overlapping a proposed Fur repressor-binding site and demonstrated iron regulation at the transcriptional level. Originally published Journal of Bacteriology, Vol. 178, No. 16, Aug 1996
Subject
Date
1996-08
Citation:
APA:
Kang, Ho Young, & Brickman, Timothy J., & Beaumont, Fiona C., & Armstrong, Sandra K.. (August 1996).
Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes..
Journal of Bacteriology,
178(16),
4877-
4884. Retrieved from
http://hdl.handle.net/10342/3319
MLA:
Kang, Ho Young, and Brickman, Timothy J., and Beaumont, Fiona C., and Armstrong, Sandra K..
"Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.". Journal of Bacteriology.
178:16. (4877-4884),
August 1996.
November 29, 2023.
http://hdl.handle.net/10342/3319.
Chicago:
Kang, Ho Young and Brickman, Timothy J. and Beaumont, Fiona C. and Armstrong, Sandra K.,
"Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.," Journal of Bacteriology 178, no.
16 (August 1996),
http://hdl.handle.net/10342/3319 (accessed
November 29, 2023).
AMA:
Kang, Ho Young, Brickman, Timothy J., Beaumont, Fiona C., Armstrong, Sandra K..
Identification and characterization of iron-regulated Bordetella pertussis alcaligin siderophore biosynthesis genes.. Journal of Bacteriology.
August 1996;
178(16):
4877-4884.
http://hdl.handle.net/10342/3319. Accessed
November 29, 2023.
Collections
Publisher
East Carolina University