E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin
Author
Kim, Hangun; Oh, Minsoo; Lu, Qun; Kim, Kwonseop
Abstract
δ-Catenin is a member of the p120-catenin subfamily of armadillo proteins. Here, we describe
distinctive features of δ-catenin localization and its association with E-cadherin in HEK293 epithelial
cells. In HEK293 cells maintained in low cell densities, approximately 15% of cells overexpressing
δ-catenin showed dendrite-like process formation, but there was no detectable change in RhoA
activity. In addition, δ-catenin was localized mainly in the cytoplasm and was associated with
p190RhoGEF. However, at high cell densities, δ-catenin localization was shifted to the plasma
membrane. The association of δ-catenin with E-cadherin was strengthened, whereas its interaction
with p190RhoGEF was weakened. In mouse embryonic fibroblast cell, ectopic expression of Ecadherin
decreased the effect of δ-catenin on the reduction of RhoA activity as well as on dendritelike
process formation. These results suggest that δ-catenin is more dominantly bound to E-cadherin
than to p190RhoGEF, and that δ-catenin’s function is dependent on its cellular binding partner. Originally published Biochem Biophys Res Commun, Vol. 377, No. 2, Dec 2008
Subject
Date
2008-12-12
Citation:
APA:
Kim, Hangun, & Oh, Minsoo, & Lu, Qun, & Kim, Kwonseop. (December 2008).
E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin.
Biochemical and Biophysical Research Communications,
377(2),
636-
641. Retrieved from
http://hdl.handle.net/10342/3322
MLA:
Kim, Hangun, and Oh, Minsoo, and Lu, Qun, and Kim, Kwonseop.
"E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin". Biochemical and Biophysical Research Communications.
377:2. (636-641),
December 2008.
December 11, 2023.
http://hdl.handle.net/10342/3322.
Chicago:
Kim, Hangun and Oh, Minsoo and Lu, Qun and Kim, Kwonseop,
"E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin," Biochemical and Biophysical Research Communications 377, no.
2 (December 2008),
http://hdl.handle.net/10342/3322 (accessed
December 11, 2023).
AMA:
Kim, Hangun, Oh, Minsoo, Lu, Qun, Kim, Kwonseop.
E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin. Biochemical and Biophysical Research Communications.
December 2008;
377(2):
636-641.
http://hdl.handle.net/10342/3322. Accessed
December 11, 2023.
Collections
Publisher
East Carolina University