Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
Author
Magge, Anil; Granger, Amanda C.; Wahome, Paul G.; Setlow, Barbara; Vepachedu, Venkata R.; Loshon, Charles A.; Peng, Lixin; Chen, De; Li, Yong-Qing; Setlow, Peter
Abstract
Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these spores as well as the DPA content in individual spores were measured. Levels of some other small molecules in DPA-less spores were also measured. These studies have allowed the following conclusions. (i) Spores with no DPA or low DPA levels that lack either the cortex-lytic enzyme (CLE) SleB or the receptors that respond to nutrient germinants could be isolated but were unstable and spontaneously initiated early steps in spore germination. (ii) Spores that lacked SleB and nutrient germinant receptors and also had low DPA levels were more stable. (iii) Spontaneous germination of spores with no DPA or low DPA levels was at least in part via activation of SleB. (iv) The other redundant CLE, CwlJ, was activated only by the release of high levels of DPA from spores. (v) Low levels of DPA were sufficient for the viability of spores that lacked most / -type small, acid-soluble spore proteins. (vi) DPA levels accumulated in spores prepared in low-DPA-containing media varied greatly between individual spores, in contrast to the presence of more homogeneous DPA levels in individual spores made in media with high DPA concentrations. (vii) At least the great majority of spores of several spoVF strains that contained no DPA also lacked other major spore small molecules and had gone through some of the early reactions in spore germination. Originally published Journal of Bacteriology, Vol. 190, No. 14, July 2008
Subject
Date
2008-07
Citation:
APA:
Magge, Anil, & Granger, Amanda C., & Wahome, Paul G., & Setlow, Barbara, & Vepachedu, Venkata R., & Loshon, Charles A., & Peng, Lixin, & Chen, De, & Li, Yong-Qing, & Setlow, Peter. (July 2008).
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis.
Journal of Bacteriology,
190(14),
4798-
4807. Retrieved from
http://hdl.handle.net/10342/3347
MLA:
Magge, Anil, and Granger, Amanda C., and Wahome, Paul G., and Setlow, Barbara, and Vepachedu, Venkata R., and Loshon, Charles A., and Peng, Lixin, and Chen, De, and Li, Yong-Qing, and Setlow, Peter.
"Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis". Journal of Bacteriology.
190:14. (4798-4807),
July 2008.
September 21, 2023.
http://hdl.handle.net/10342/3347.
Chicago:
Magge, Anil and Granger, Amanda C. and Wahome, Paul G. and Setlow, Barbara and Vepachedu, Venkata R. and Loshon, Charles A. and Peng, Lixin and Chen, De and Li, Yong-Qing and Setlow, Peter,
"Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis," Journal of Bacteriology 190, no.
14 (July 2008),
http://hdl.handle.net/10342/3347 (accessed
September 21, 2023).
AMA:
Magge, Anil, Granger, Amanda C., Wahome, Paul G., Setlow, Barbara, Vepachedu, Venkata R., Loshon, Charles A., Peng, Lixin, Chen, De, Li, Yong-Qing, Setlow, Peter.
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis. Journal of Bacteriology.
July 2008;
190(14):
4798-4807.
http://hdl.handle.net/10342/3347. Accessed
September 21, 2023.
Collections
Publisher
East Carolina University