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    Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.

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    Author
    Rogers, Marc B.; Parker, Anita C.; Smith, C. Jeffrey
    Abstract
    Bacteroides frgiglis CS30 is a clinical isolate resistant to high concentrations of benzylpenicillin and cephaloridine but not to cephamycin or penem antibiotics. beta-Lactam resistance is mediated by a chromosomally encoded cephalosporinase produced at a high level. The gene encoding this beta-lactamase was cloned from genomic libraries constructed in Escherichia coli and then mated with B. fragilis 638 for identification of ampicillin-resistant (Apr) strains. Apr transconjugants contained a nitrocefin-reactive protein with the physical and enzymatic properties of the original CS30 isolate. The beta-lactamase gene (cepA) was localized by deletion analysis and subcloned, and its nucleotide sequence was determined. The 903-bp cepA open reading frame encoded a 300-amino-acid precursor protein (predicted molecular mass, 34,070 Da). A 13-lactamase-deficient mutant strain of B. fiugilis 638 was constructed by insertional inactivation with the cepA gene of CS30, demonstrating strict functional homology between these chromosomal beta-lactamase genes. An extensive comparison of the CepA protein sequence by alignment with other beta-lactamases revealed the strict conservation of at least four elements common to Ambler class A. A further comparison of the CepA protein sequence with protein sequences of beta-lactamases from two other Bacteroides species indicated that they constitute their own distinct subgroup of class A beta-lactamases. Originally published Antimicrobial Agents and Chemotherapy, Vol. 37, No. 11, Nov 1993
    URI
    http://hdl.handle.net/10342/3372
    Subject
     Bacteroides fragilis; Beta-lactamase; Cephalosporinase 
    Date
    1993-11
    Citation:
    APA:
    Rogers, Marc B., & Parker, Anita C., & Smith, C. Jeffrey. (November 1993). Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.. Antimicrobial Agents and Chemotherapy, (37:11), p.2391-2400. Retrieved from http://hdl.handle.net/10342/3372

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Rogers, Marc B., and Parker, Anita C., and Smith, C. Jeffrey. "Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.". Antimicrobial Agents and Chemotherapy. 37:11. (2391-2400.), November 1993. March 07, 2021. http://hdl.handle.net/10342/3372.
    Chicago:
    Rogers, Marc B. and Parker, Anita C. and Smith, C. Jeffrey, "Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.," Antimicrobial Agents and Chemotherapy 37, no. 11 (November 1993), http://hdl.handle.net/10342/3372 (accessed March 07, 2021).
    AMA:
    Rogers, Marc B., Parker, Anita C., Smith, C. Jeffrey. Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.. Antimicrobial Agents and Chemotherapy. November 1993; 37(11) 2391-2400. http://hdl.handle.net/10342/3372. Accessed March 07, 2021.
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    • Microbiology and Immunology
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    East Carolina University

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