The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities
Author
Petersen, Steen V.; Olsen, Dorte Aa.; Kenney, John M.; Oury, Tim D.; Valnickova, Zuzana; Thogersen, Ida B.; Crapo, James D.; Enghild, Jan J.
Abstract
The C-terminal region of EC-SOD (extracellular superoxide dismutase) mediates the binding to both heparin/heparan sulphate and type I collagen. A mutation (Arg213→Gly; R213G) within this extracellular matrix-binding region has recently been implicated in the development of heart disease. This relatively common mutation affects the heparin affinity, and the concentration of EC-SOD in the plasma of R213G homozygous individuals is increased 10- to 30-fold. In the present study we confirm, using R213G EC-SOD purified from a homozygous individual, that the heparin affinity is reduced. Significantly, the collagen affinity of the R213G EC-SOD variant was similarly affected and both the heparin and collagen affinitieswere reduced by 12-fold. Structural analysis of synthetic extracellularmatrix-binding regions suggests that the mutation alters the secondary structure.We conclude that the increased concentration of EC-SOD in the plasma of R213G
carriers is caused by a reduction in both heparin and collagen affinities. Originally published Biochemical Journal, Vol. 385, No. 2, Jan 2005
Date
2005-01-15
Citation:
APA:
Petersen, Steen V., & Olsen, Dorte Aa., & Kenney, John M., & Oury, Tim D., & Valnickova, Zuzana, & Thogersen, Ida B., & Crapo, James D., & Enghild, Jan J.. (January 2005).
The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities.
Biochemical Journal,
385(2),
427-
432. Retrieved from
http://hdl.handle.net/10342/3380
MLA:
Petersen, Steen V., and Olsen, Dorte Aa., and Kenney, John M., and Oury, Tim D., and Valnickova, Zuzana, and Thogersen, Ida B., and Crapo, James D., and Enghild, Jan J..
"The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities". Biochemical Journal.
385:2. (427-432),
January 2005.
April 25, 2024.
http://hdl.handle.net/10342/3380.
Chicago:
Petersen, Steen V. and Olsen, Dorte Aa. and Kenney, John M. and Oury, Tim D. and Valnickova, Zuzana and Thogersen, Ida B. and Crapo, James D. and Enghild, Jan J.,
"The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities," Biochemical Journal 385, no.
2 (January 2005),
http://hdl.handle.net/10342/3380 (accessed
April 25, 2024).
AMA:
Petersen, Steen V., Olsen, Dorte Aa., Kenney, John M., Oury, Tim D., Valnickova, Zuzana, Thogersen, Ida B., Crapo, James D., Enghild, Jan J..
The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities. Biochemical Journal.
January 2005;
385(2):
427-432.
http://hdl.handle.net/10342/3380. Accessed
April 25, 2024.
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Publisher
East Carolina University