• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Internal Medicine
    • View Item
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Internal Medicine
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.

    Thumbnail
    View/ Open
    MCF 7 breast cancer cells.pdf (5.463Mb)

    Show full item record
    
    Author
    Ways, D. Kirk; Kukoly, Cynthia A.; DeVente, James E.; Hooker, Jerry L.; Bryant, Winifred O.; Posekany, Karla J.; Fletcher, Donald J.; Cook, Paul P.; Parker, Peter J.
    Abstract
    Increased protein kinase C (PKC) activity in malignant breast tissue and positive correlations between PKC activity and expression of a more aggressive phenotype in breast cancer cell lines suggest a role for this signal transduction pathway in the pathogenesis and/or progression of breast cancer. To examine the role of PKC in the progression of breast cancer, human MCF-7 breast cancer cells were transfected with PKC-a, and a group of heterogenous cells stably over expressing PKC-a were isolated (MCF-7-PKC- a).MCF-7-PKC-a cells expressed five fold higher levels of PKC-a as compared to parental or vector-transfected MCF- 7 cels. MCF-7-PKC-a cells also displayed a substantial increase in endogenous expression of PKC-8 and decreases in expression of the novel 6- and q-PKC isoforms. MCF-7-PKC-a cells displayed an enhanced proliferative rate, anchorage-independent growth, dramatic morphologic alterations including loss of an epithelioid appearance, and increased tumorigenicity in nude mice. MCF-7-PKC- a cells exhibited a significant reduction in estrogen receptor expression and decreases in estrogen-dependent gene expression. These findings suggest that the PKC pathway may modulate progression of breast cancer to a more aggressive neoplastic process. Originally published Journal of Clinical Investigation, Vol. 95, No. 4, Apr 1995
    URI
    http://hdl.handle.net/10342/3403
    Subject
     PKC isoforms; Vimentin; Estrogen receptor; Anchorage independent; Tumorigenicity 
    Date
    1995-04
    Citation:
    APA:
    Ways, D. Kirk, & Kukoly, Cynthia A., & DeVente, James E., & Hooker, Jerry L., & Bryant, Winifred O., & Posekany, Karla J., & Fletcher, Donald J., & Cook, Paul P., & Parker, Peter J.. (April 1995). MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.. , (. Retrieved from http://hdl.handle.net/10342/3403

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Ways, D. Kirk, and Kukoly, Cynthia A., and DeVente, James E., and Hooker, Jerry L., and Bryant, Winifred O., and Posekany, Karla J., and Fletcher, Donald J., and Cook, Paul P., and Parker, Peter J.. "MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.". . . (.), April 1995. March 04, 2021. http://hdl.handle.net/10342/3403.
    Chicago:
    Ways, D. Kirk and Kukoly, Cynthia A. and DeVente, James E. and Hooker, Jerry L. and Bryant, Winifred O. and Posekany, Karla J. and Fletcher, Donald J. and Cook, Paul P. and Parker, Peter J., "MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.,"  , no. (April 1995), http://hdl.handle.net/10342/3403 (accessed March 04, 2021).
    AMA:
    Ways, D. Kirk, Kukoly, Cynthia A., DeVente, James E., Hooker, Jerry L., Bryant, Winifred O., Posekany, Karla J., Fletcher, Donald J., Cook, Paul P., Parker, Peter J.. MCF-7 breast cancer cells transfected with protein kinase C-alpha exhibit altered expression of other protein kinase C isoforms and display a more aggressive neoplastic phenotype.. . April 1995; () . http://hdl.handle.net/10342/3403. Accessed March 04, 2021.
    Collections
    • Internal Medicine
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback