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    Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase

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    Author
    Silo-Suh, Laura; Suh, Sang-Jin; Phibbs, Paul V.; Ohman, Dennis E.
    Abstract
    Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a 90% reduction in alginate production in the mucoid, CF isolate, P. aeruginosa FRD1. The main regulator of alginate, sigma-22 encoded by algT (algU), plays a small but demonstrable role in the induction of zwf expression in P. aeruginosa. However, G6PDH activity and zwf expression were higher in FRD1 strains than in PAO1 strains. In PAO1, zwf expression and G6PDH activity are known to be subject to catabolite repression by succinate. In contrast, FRD1 zwf expression and G6PDH activity were shown to be refractory to such catabolite repression. This was apparently not due to a defect in the catabolite repression control (Crc) protein. Such relaxed control of zwf was found to be common among several examined CF isolates but was not seen in other strains of clinical and environmental origin. Two sets of clonal isolates from individual CF patient indicated that the resident P. aeruginosa strain underwent an adaptive change that deregulated zwf expression. We hypothesized that high-level, unregulated G6PDH activity provided a survival advantage to P. aeruginosa within the lung environment. Interestingly, zwf expression in P. aeruginosa was shown to be required for its resistance to human sputum. This study illustrates that adaptation to the CF pulmonary environment by P. aeruginosa can include altered regulation of basic metabolic activities, including carbon catabolism. Originally published Journal of Bacteriology, Vol. 187, No. 22, Nov 2005
    URI
    http://hdl.handle.net/10342/3406
    Subject
     Pseudomonas aeruginosa; Cystic Fibrosis; Zwf deregulation 
    Date
    2005-11
    Citation:
    APA:
    Silo-Suh, Laura, & Suh, Sang-Jin, & Phibbs, Paul V., & Ohman, Dennis E.. (November 2005). Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase. Journal of Bacteriology, (187:22), p.7561-7568. Retrieved from http://hdl.handle.net/10342/3406

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Silo-Suh, Laura, and Suh, Sang-Jin, and Phibbs, Paul V., and Ohman, Dennis E.. "Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase". Journal of Bacteriology. 187:22. (7561-7568.), November 2005. August 16, 2022. http://hdl.handle.net/10342/3406.
    Chicago:
    Silo-Suh, Laura and Suh, Sang-Jin and Phibbs, Paul V. and Ohman, Dennis E., "Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase," Journal of Bacteriology 187, no. 22 (November 2005), http://hdl.handle.net/10342/3406 (accessed August 16, 2022).
    AMA:
    Silo-Suh, Laura, Suh, Sang-Jin, Phibbs, Paul V., Ohman, Dennis E.. Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase. Journal of Bacteriology. November 2005; 187(22) 7561-7568. http://hdl.handle.net/10342/3406. Accessed August 16, 2022.
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    • Microbiology and Immunology
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    East Carolina University

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