A Long Helix from the Central Region of Smooth Muscle Caldesmon
Author
Wang, C.-L. Albert; Chalovich, Joseph; Graceffa, Philip; Lu, Renne C.; Mabuchi, Katsuhide; Stafford, Walter F.
Abstract
The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic
properties and the electron microscopic images suggest that CT54 is an elongated (35 nm), monomeric molecule. The circular dichroic spectrum yields an overall α-helical content of 55–58%. These results are consistent with the model that the middle portion of CT54 forms a long stretch of single-stranded α-helix. Such a structure, if it in fact exists, is thought to be stabilized by numerous salt bridges between charged residues at positions i and i+4. The structural characteristics of this fragment not only represent an unusual protein configuration but also provide information about the functional role of caldesmon in smooth muscle contraction. Originally published Journal of Biological Chemistry, Vol. 266, No. 21, July 1991
Subject
Date
1991-07-25
Citation:
APA:
Wang, C.-L. Albert, & Chalovich, Joseph, & Graceffa, Philip, & Lu, Renne C., & Mabuchi, Katsuhide, & Stafford, Walter F.. (July 1991).
A Long Helix from the Central Region of Smooth Muscle Caldesmon.
Journal of Biological Chemistry,
266(21),
13958-
13963. Retrieved from
http://hdl.handle.net/10342/3413
MLA:
Wang, C.-L. Albert, and Chalovich, Joseph, and Graceffa, Philip, and Lu, Renne C., and Mabuchi, Katsuhide, and Stafford, Walter F..
"A Long Helix from the Central Region of Smooth Muscle Caldesmon". Journal of Biological Chemistry.
266:21. (13958-13963),
July 1991.
September 22, 2023.
http://hdl.handle.net/10342/3413.
Chicago:
Wang, C.-L. Albert and Chalovich, Joseph and Graceffa, Philip and Lu, Renne C. and Mabuchi, Katsuhide and Stafford, Walter F.,
"A Long Helix from the Central Region of Smooth Muscle Caldesmon," Journal of Biological Chemistry 266, no.
21 (July 1991),
http://hdl.handle.net/10342/3413 (accessed
September 22, 2023).
AMA:
Wang, C.-L. Albert, Chalovich, Joseph, Graceffa, Philip, Lu, Renne C., Mabuchi, Katsuhide, Stafford, Walter F..
A Long Helix from the Central Region of Smooth Muscle Caldesmon. Journal of Biological Chemistry.
July 1991;
266(21):
13958-13963.
http://hdl.handle.net/10342/3413. Accessed
September 22, 2023.
Collections
Publisher
East Carolina University