delta-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles
Zeng, Yan; Abdallah, Agustin; Lu, Jian-Ping; Wang, Tao; Chen, Yan-Hua; Terrian, David M.; Kim, Kwonseop; Lu, Qun
Background: delta-Catenin is a unique member of delta-catenin/armadillo domain superfamily proteins and its primary expression is restricted to the brain. However, delta-catenin is upregulated in human prostatic adenocarcinomas, although the effects of delta-catenin overexpression in prostate cancer are unclear. We hypothesized that delta-catenin plays a direct role in prostate cancer progression by altering gene profiles of cell cycle regulation and cell survival. Results: We employed gene transfection and small interfering RNA to demonstrate that increased delta-catenin expression promoted, whereas its knockdown suppressed prostate cancer cell viability. delta-Catenin promoted prostate cancer cell colony formation in soft agar as well as tumor xenograft growth in nude mice. Deletion of either the amino-terminal or carboxyl-terminal sequences outside the armadillo domains abolished the tumor promoting effects of delta-catenin. Quantitative RT2 Profiler™ PCR Arrays demonstrated gene alterations involved in cell cycle and survival regulation. delta-Catenin overexpression upregulated cyclin D1 and cdc34, increased phosphorylated histone-H3, and promoted the entry of mitosis. In addition, delta-catenin overexpression resulted in increased expression of cell survival genes Bcl-2 and survivin while reducing the cell cycle inhibitor p21Cip1. Conclusion: Taken together, our studies suggest that at least one consequence of an increased expression of delta-catenin in human prostate cancer is the alteration of cell cycle and survival gene profiles, thereby promoting tumor progression. Originally published Molecular Cancer, Vol. 8, No. 19, Mar 2009
Zeng, Yan, & Abdallah, Agustin, & Lu, Jian-Ping, & Wang, Tao, & Chen, Yan-Hua, & Terrian, David M., & Kim, Kwonseop, & Lu, Qun. (March 2009). delta-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles. Molecular Cancer, (8:19), p.1-11. Retrieved from http://hdl.handle.net/10342/3414
Zeng, Yan, and Abdallah, Agustin, and Lu, Jian-Ping, and Wang, Tao, and Chen, Yan-Hua, and Terrian, David M., and Kim, Kwonseop, and Lu, Qun. "delta-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles". Molecular Cancer. 8:19. (1-11.), March 2009. October 26, 2020. http://hdl.handle.net/10342/3414.
Zeng, Yan and Abdallah, Agustin and Lu, Jian-Ping and Wang, Tao and Chen, Yan-Hua and Terrian, David M. and Kim, Kwonseop and Lu, Qun, "delta-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles," Molecular Cancer 8, no. 19 (March 2009), http://hdl.handle.net/10342/3414 (accessed October 26, 2020).
Zeng, Yan, Abdallah, Agustin, Lu, Jian-Ping, Wang, Tao, Chen, Yan-Hua, Terrian, David M., Kim, Kwonseop, Lu, Qun. delta-Catenin promotes prostate cancer cell growth and progression by altering cell cycle and survival gene profiles. Molecular Cancer. March 2009; 8(19) 1-11. http://hdl.handle.net/10342/3414. Accessed October 26, 2020.
East Carolina University