Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice
Author
Shibata, Yoshimi; Honda, Ikuro; Justice, J. Paul; Van Scott, Michael R.; Nakamura, Reiko M.; Myrvik, Quentin N.
Abstract
Treatment of mice with heat-killed (HK) Mycobacterium bovis BCG or 1- to 10-μm chitin particles (nonantigenic N-acetyl-d-glucosamine polymers) is known to induce innate immune responses, including gamma interferon (IFN-γ) production, which plays a Th1 adjuvant role. However, HK BCG further induces prostaglandin E2-releasing spleen macrophages (Mφ) (PGE2-Mφ), which potentially inhibit Th1 adjuvant activities. We found that chitin particles did not induce PGE2-Mφ formation. To further assess whether chitin has Th1 adjuvant effects, interleukin-10 (IL-10)-knockout (KO) mice and their wild-type (WT, C57BL/6) controls were immunized with a 30-kDa MPB-59 mycobacterial protein mixed with chitin. Immunization with MPB-59 alone induced Th2 responses, characterized by increases in total serum immunoglobulin E (IgE) and specific serum IgG1 levels and spleen Th2 cells producing IL-4, IL-5, and IL-10. No IFN-γ-producing spleen Th1 cells, specific serum IgG2a, or delayed-type hypersentivity (DTH) footpad reactions were detected. On the other hand, chitin–MPB-59 immunization significantly increased spleen Th1 responses, DTH reaction, and serum IgG2a levels along with decreases of Th2 responses. The magnitude of these Th1 adjuvant effects was greater in IL-10-KO mice than in WT mice. In contrast, immunization with HK BCG–MPB-59 showed little or no Th1 adjuvant effect. These data indicate that chitin has a unique Th1 adjuvant effect on the development of Th1 immunity against a mycobacterial antigen. IL-10 down-regulates the adjuvant effect of chitin. Originally published Infection and Immunity, Vol. 69, No. 10, Oct 2001
Date
2001-10
Citation:
APA:
Shibata, Yoshimi, & Honda, Ikuro, & Justice, J. Paul, & Van Scott, Michael R., & Nakamura, Reiko M., & Myrvik, Quentin N.. (October 2001).
Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice.
Infection and Immunity,
69(10),
6123-
6130. Retrieved from
http://hdl.handle.net/10342/3422
MLA:
Shibata, Yoshimi, and Honda, Ikuro, and Justice, J. Paul, and Van Scott, Michael R., and Nakamura, Reiko M., and Myrvik, Quentin N..
"Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice". Infection and Immunity.
69:10. (6123-6130),
October 2001.
November 29, 2023.
http://hdl.handle.net/10342/3422.
Chicago:
Shibata, Yoshimi and Honda, Ikuro and Justice, J. Paul and Van Scott, Michael R. and Nakamura, Reiko M. and Myrvik, Quentin N.,
"Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice," Infection and Immunity 69, no.
10 (October 2001),
http://hdl.handle.net/10342/3422 (accessed
November 29, 2023).
AMA:
Shibata, Yoshimi, Honda, Ikuro, Justice, J. Paul, Van Scott, Michael R., Nakamura, Reiko M., Myrvik, Quentin N..
Th1 Adjuvant N-Acetyl-d-Glucosamine Polymer Up-Regulates Th1 Immunity but Down-Regulates Th2 Immunity against a Mycobacterial Protein (MPB-59) in Interleukin-10-Knockout and Wild-Type Mice. Infection and Immunity.
October 2001;
69(10):
6123-6130.
http://hdl.handle.net/10342/3422. Accessed
November 29, 2023.
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Publisher
East Carolina University