• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Academic Affairs
    • Thomas Harriot College of Arts and Sciences
    • Biology
    • View Item
    •   ScholarShip Home
    • Academic Affairs
    • Thomas Harriot College of Arts and Sciences
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Posttranslational modifications in the CP43 subunit of photosystem II

    Thumbnail
    View/ Open
    Posttranslational modifications CP43 subunit.pdf (350.2Kb)

    Show full item record
    Author
    Anderson, Lorraine B.; Maderia, Melissa; Ouellette, Anthony J. A.; Putnam-Evans, Cindy; Higgins, LeeAnn; Krick, Thomas; MacCoss, MIchael J.; Lim, Hango; Yates, John R. III; Barry, Bridgette A.
    Abstract
    Photosystem II (PSII) catalyzes the light-driven oxidation of water and the reduction of plastoquinone; the oxidation of water occurs at a cluster of four manganese. The PSII CP43 subunit functions in light harvesting, and mutations in the fifth luminal loop (E) of CP43 have established its importance in PSII structure and or assembly [Kuhn, M. G. & Vermaas, V. F. J. (1993) Plant Mol. Biol. 23, 123–133]. The sequence A350PWLEPLR357 in luminal loop E is conserved in CP43 genes from 50 organisms. To map important posttranslational modifications in this sequence, tandem mass spectrometry (MS MS) was used. These data show that the indole side chain of Trp-352 is posttranslationally modified to give mass shifts of +4, +16, and +18 daltons. The masses of the modifications suggest that the tryptophan is modified to kynurenine (+4), a keto- amino- hydroxy- (+16) derivative, and a dihydro-hydroxy- (+18) derivative of the indole side chain. Peptide synthesis and MS MS confirmed the kynurenine assignment. The +16 and +18 tryptophan modifications may be intermediates formed during the oxidative cleavage of the indole ring to give kynurenine. The sitedirected mutations, W352C, W352L, and W352A, exhibit an increased rate of photoinhibition relative to wild type. We hypothesize that Trp-352 oxidative modifications are a byproduct of PSII water-splitting or electron transfer reactions and that these modifications target PSII for turnover. As a step toward understanding the tertiary structure of this CP43 peptide, structural modeling was performed by using molecular dynamics. Originally published Proceedings of the National Academy of Sciences, Vol. 99, No. 23, Nov 2002
    URI
    http://hdl.handle.net/10342/3436
    Subject
     Mass spectrometry; Collision-induced dissociation; Tryptophan; Kynurenine; Photoinhibition 
    Date
    2002-11-12
    Citation:
    APA:
    Anderson, Lorraine B., & Maderia, Melissa, & Ouellette, Anthony J. A., & Putnam-Evans, Cindy, & Higgins, LeeAnn, & Krick, Thomas, & MacCoss, MIchael J., & Lim, Hango, & Yates, John R. III, & Barry, Bridgette A.. (November 2002). Posttranslational modifications in the CP43 subunit of photosystem II. , (. Retrieved from http://hdl.handle.net/10342/3436

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Anderson, Lorraine B., and Maderia, Melissa, and Ouellette, Anthony J. A., and Putnam-Evans, Cindy, and Higgins, LeeAnn, and Krick, Thomas, and MacCoss, MIchael J., and Lim, Hango, and Yates, John R. III, and Barry, Bridgette A.. "Posttranslational modifications in the CP43 subunit of photosystem II". . . (.), November 2002. March 04, 2021. http://hdl.handle.net/10342/3436.
    Chicago:
    Anderson, Lorraine B. and Maderia, Melissa and Ouellette, Anthony J. A. and Putnam-Evans, Cindy and Higgins, LeeAnn and Krick, Thomas and MacCoss, MIchael J. and Lim, Hango and Yates, John R. III and Barry, Bridgette A., "Posttranslational modifications in the CP43 subunit of photosystem II,"  , no. (November 2002), http://hdl.handle.net/10342/3436 (accessed March 04, 2021).
    AMA:
    Anderson, Lorraine B., Maderia, Melissa, Ouellette, Anthony J. A., Putnam-Evans, Cindy, Higgins, LeeAnn, Krick, Thomas, MacCoss, MIchael J., Lim, Hango, Yates, John R. III, Barry, Bridgette A.. Posttranslational modifications in the CP43 subunit of photosystem II. . November 2002; () . http://hdl.handle.net/10342/3436. Accessed March 04, 2021.
    Collections
    • Biology
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback