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    Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster

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    Author
    Hunter, Chad Michael
    Abstract
    DNA is the genetic material for all living organisms which is constantly being unpackaged, replicated and repackaged. The replication of this genetic material involves numerous different proteins; however, DNA polymerase delta (pol [delta]) carries much of the load by replicating a major portion of the genome in both leading and lagging strand synthesis. Using the model organism, Drosophila melanogaster, we investigate two novel mutations in two different evolutionary conserved regions. One region corresponds with the polymerase's ability to polymerize new nucleotides onto an existing strand of DNA. The other region corresponds with the polymerase's ability to proofread in the 3' to 5' direction.  These two mutants, both homozygous lethal and recessive, show interesting phenotypes with a delay in S-phase, numerous chromosome aberrations, defects in endoreplication and possible protection form DNA damage. Using these two mutants, these two domains can be further characterized. By understanding how pol [delta] functions in an in vivo setting, we can apply this knowledge to the mechanics of cancer biology in humans, another multicelluar organism, and inform new therapies to treat it.  
    URI
    http://hdl.handle.net/10342/3627
    Subject
     Genetics; Biology; Biology, Molecular; DNA polymerase delta; DNA replication; Biology, Genetics; Molecular biology 
    Date
    2011
    Citation:
    APA:
    Hunter, Chad Michael. (January 2011). Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/3627.)

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    MLA:
    Hunter, Chad Michael. Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster. Master's Thesis. East Carolina University, January 2011. The Scholarship. http://hdl.handle.net/10342/3627. February 26, 2021.
    Chicago:
    Hunter, Chad Michael, “Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster” (Master's Thesis., East Carolina University, January 2011).
    AMA:
    Hunter, Chad Michael. Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster [Master's Thesis]. Greenville, NC: East Carolina University; January 2011.
    Collections
    • Biology
    • Master's Theses
    Publisher
    East Carolina University

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