• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • ECU Main Campus
    • Thomas Harriot College of Arts and Sciences
    • Biology
    • View Item
    •   ScholarShip Home
    • ECU Main Campus
    • Thomas Harriot College of Arts and Sciences
    • Biology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Effect of MicroRNA-34c on Regenerative Axon Growth in Dorsal Root Ganglion Neurons of the Peripheral Nervous System

    Thumbnail
    View/ Open
    Raafat_ecu_0600M_10583.pdf (4.767Mb)
    MicroRNa-biomachinerypaper.pdf (2.303Mb)

    Show full item record
    Author
    Raafat, Mohamed Hussien
    Abstract
    The peripheral nervous system is unique in its ability to regenerate axons after injury. Our lab previously assessed the miRNA expression levels of the regenerating sciatic nerve which indicated miR-34c up-regulation. MicroRNAs are non-protein coding small RNA molecules, ~20 nt in length, that have been shown to control many basic cellular processes by post-transcriptional regulation. The purpose of this study was to identify the effect of miR-34c on regenerating axons of DRG neurons. 9 to 12 week CD-1 male mice used for this study were subjected to sciatic nerve crush lesion. Five days after surgery, animals were sacrificed, and DRGs were dissected and dissociated physically and enzymatically. DRG neurons were then grouped as follows: 1) non-transfected, 2) miR-34c mimic, 3) miR-34c inhibitor, 4) mimic negative control, and 5) inhibitor negative control. Cells were grown for 48 hours on glass cover slips, fixed, and incubated with primary beta-tubulin III antibody and with secondary antibody conjugated with a fluorophore. Cells were viewed on an inverted fluorescent microscope, and images were captured using a digital camera. Axon length and branches were measured using ImageJ. Inhibition of miR-34c showed a 27% increase in axon length for naive cultures (p<0.05), and a 23% increase in regenerating cultures (p<0.05), when compared to the inhibitor negative control. Also, a potential miR-34c target named Neuron Navigator 1 (NAV1) expression showed 43% increase after inhibition (p<0.05). Over-expression of miR-34c showed a 16% decrease in branching of regenerating cultures (p<0.05), as well as a 52% decrease in NAV1 expression. In conclusion, miR-34c inhibitor promoted axon growth in regenerating cultures resulting in longer axons and increased NAV1 expression. Over-expression of miR-34c produced a degenerative response in regenerating neurons resulting in decreased branching and decreased NAV1 expression.  
    URI
    http://hdl.handle.net/10342/3834
    Subject
     Physiology; Bioinformatics; Genetics; Axons; Drg; MicroRNA-34c; Mir-34c; Nerve regeneration; Peripheral nervous system; Biology, Genetics; Biology, Physiology 
    Date
    2011
    Citation:
    APA:
    Raafat, Mohamed Hussien. (January 2011). Effect of MicroRNA-34c on Regenerative Axon Growth in Dorsal Root Ganglion Neurons of the Peripheral Nervous System (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/3834.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Raafat, Mohamed Hussien. Effect of MicroRNA-34c on Regenerative Axon Growth in Dorsal Root Ganglion Neurons of the Peripheral Nervous System. Master's Thesis. East Carolina University, January 2011. The Scholarship. http://hdl.handle.net/10342/3834. September 26, 2023.
    Chicago:
    Raafat, Mohamed Hussien, “Effect of MicroRNA-34c on Regenerative Axon Growth in Dorsal Root Ganglion Neurons of the Peripheral Nervous System” (Master's Thesis., East Carolina University, January 2011).
    AMA:
    Raafat, Mohamed Hussien. Effect of MicroRNA-34c on Regenerative Axon Growth in Dorsal Root Ganglion Neurons of the Peripheral Nervous System [Master's Thesis]. Greenville, NC: East Carolina University; January 2011.
    Collections
    • Biology
    • Master's Theses
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback