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    Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development

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    Author
    Nagchowdhuri, Partha S.
    Abstract
    The different mechanisms involved in vertebrate synovial joint development are actively  being uncovered. A variety of studies have thus far discovered the involvement of several large  molecules in the processes of joint development and cavitation. Such molecules include  glycosaminoglycans, glycoproteins, proteoglycans, growth factors, and adhesive molecules. The  coordinated spatiotemporal expressions of these molecules, among them chondroitin-sulfate  proteoglycans (CSPG), are believed to play a crucial role in synovial joint formation. A previous  study has shown that isoforms of the CSPG versican are abundantly expressed in the developing  chick synovial joint during progressive stages of embryonic development (Shepard et al. 2007).  The purpose of this study is to better understand the importance of versican in the developing  limb joints by inhibiting its expression. We employed adenoviral-mediated RNA interference  and inovo microinjection techniques to knock down endogenous versican protein levels. Results  show an average reduction of 14% in the non-chondrogenic space of the joint interzone of day 10  embryos in response to versican knockdown that was coupled with attenuated expressions of key  versican associating molecules such as hyaluronan, tenascin, CD44 and link protein. Impacts of  versican knockdown also resulted in lowered collagen II expression among articular  chondrocytes implying possible delays in chondrogenesis, or early transition toward an  osteogenic fate.  
    URI
    http://hdl.handle.net/10342/4090
    Subject
     Biology, Molecular; Molecular biology 
    Date
    2012
    Citation:
    APA:
    Nagchowdhuri, Partha S.. (January 2012). Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/4090.)

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    MLA:
    Nagchowdhuri, Partha S.. Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development. Master's Thesis. East Carolina University, January 2012. The Scholarship. http://hdl.handle.net/10342/4090. May 19, 2022.
    Chicago:
    Nagchowdhuri, Partha S., “Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development” (Master's Thesis., East Carolina University, January 2012).
    AMA:
    Nagchowdhuri, Partha S.. Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development [Master's Thesis]. Greenville, NC: East Carolina University; January 2012.
    Collections
    • Biology
    • Master's Theses
    Publisher
    East Carolina University

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