• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Health Sciences Campus
    • Brody School of Medicine
    • Biomedical Sciences
    • View Item
    •   ScholarShip Home
    • Health Sciences Campus
    • Brody School of Medicine
    • Biomedical Sciences
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats

    Thumbnail
    View/ Open
    Holland_ecu_0600O_11215.pdf (3.150Mb)

    Show full item record
    Author
    Holland, Nathan A.
    Abstract
    The uses of engineered nanomaterials have expanded in biomedical technology and consumer manufacturing. Exposure to particulate matter has been demonstrated to negatively influence cardiovascular health and expand myocardial infarction. Furthermore, pulmonary exposure to various engineered nanomaterials has, likewise, demonstrated the ability to exacerbate cardiac ischemia reperfusion (I/R) injury. We hypothesized that pulmonary exposure to AgNP induces cardiovascular toxicity in the form of expanded I/R injury, electrical dysfunction and inducing a persistent increase in circulating proinflammatory cytokines. To test this hypothesis, we exposed male SD rats to an intratracheal (IT) instillation of 200 µg of 20 or 110 nm polyvinylprryolidone (PVP) or citrate capped AgNP, in 200 ul of the respective PVP or citrate vehicle. Serum samples were collected prior to instillation and 1, 3, 6, 24, 48, 72, and 168 hours following instillation. Serum samples were analyzed by multiplex assay for concentrations of: G-CSF, GM-CSF, MIP-1a, IL -1b, IL-2, IL-5, IL-6, IL-10, IL-13, IL-17a, IL-18, MCP-1, IFNy, RANTES, and TNFa. Twenty four and 168 hours after IT exposure, cardiac ischemia was induced by left anterior descending coronary artery ligation for 20 minutes followed by 2 hours of reperfusion. Intraoperative ECG was monitored throughout cardiac I/R surgery for heart rate (HR), PR interval, and QT interval. To test the impact of silver ion exposure on cardiac I/R injury we administered 200 ul of 0.01 mg/mL, 0.1 mg/mL, or 1 mg/mL silver acetate (AgAc) and induced cardiac I/R 24 hours later. Intratracheal instillation of AgNP resulted in expansion of I/R injury for both sizes of citrate and PVP capped AgNP at both 24 hours and 168 following instillation; exposure to 0.1 and 1 mg/mL AgAc also resulted in expansion of I/R injury. Intratracheal instillation of AgNP did not result in increased serum concentrations of selected proinflammatory cytokines, however post I/R serum levels of IL-2, IL-6, and IL-18 were significantly elevated in rats exposed to 20 nm PVP capped AgNP compared to vehicle controls at 24 hours post instillation. Instillation of AgNP had no impact on HR or QT interval. However, exposure to 20 nm AgNP resulted in a differential prolongation or shortening of PR interval during reperfusion based on capping agent. In conclusion IT instillation of AgNP exacerbates cardiac I/R injury 24 and 168 hours following instillation, without inducing a strong systemic inflammatory response or electrical dysfunction. Exposure to AgNP may result in a sensitization of the immune system in response to a secondary insult (e.g., cardiac I/R) which are largely correlated with capping agents and particle size and may drive expansion of I/R injury at 24 and 168 hours following IT instillation of AgNP.  
    URI
    http://hdl.handle.net/10342/4563
    Subject
     Physiology; Toxicology; Cardiac; Inflammation; Ischemia; Nanomaterial; Reperfusion; Silver; Biology, Physiology 
    Date
    2014
    Citation:
    APA:
    Holland, Nathan A.. (January 2014). Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats (Master's Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/4563.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Holland, Nathan A.. Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats. Master's Thesis. East Carolina University, January 2014. The Scholarship. http://hdl.handle.net/10342/4563. September 26, 2023.
    Chicago:
    Holland, Nathan A., “Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats” (Master's Thesis., East Carolina University, January 2014).
    AMA:
    Holland, Nathan A.. Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats [Master's Thesis]. Greenville, NC: East Carolina University; January 2014.
    Collections
    • Biomedical Sciences
    • Master's Theses
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback