Catabolite repression control (Crc) gene and Pseudomonas virulence
Phibbs, Paul V.; Collier, David N.; Hager, Paul W.
The present invention relates to a method of screening for compounds that inhibit the virulence of Pseudomonas bacteria and comprises the steps of: providing a culture medium comprising Pseudomonas bacteria, administering a test compound to said bacteria, and then detecting the presence or absence of inhibition of the catabolite repression control (Crc) protein in the bacteria. The inhibition of the Crc protein indicates that the compound has antivirulence activity against Pseudomonas bacteria. Antisense oligonucleotides that inhibit expression of the Crc protein in a Pseudomonas bacteria and is nuclease resistant are also disclosed. Antivirulence compounds and the uses thereof in treating Pseudomonas infections are also disclosed.