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Identification and Characterization of alcR, a Gene Encoding an AraC-Like Regulator of Alcaligin Siderophore Biosynthesis and Transport in Bordetella pertussis and Bordetella bronchiseptica
(East Carolina University, 1998-02)
A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin ...
Transcriptional Analysis of the Bordetella Alcaligin Siderophore Biosynthesis Operon
(East Carolina University, 1998-02)
The alc gene cluster of Bordetella pertussis includes three genes, alcA, alcB, and alcC, which are involved in alcaligin siderophore biosynthesis in response to iron starvation. The production of AlcA, AlcB, and AlcC in ...
The Transfer Origin for Bacteroides Mobilizable Transposon Tn4555 Is Related to a Plasmid Family from Gram-Positive Bacteria
(East Carolina University, 1998-01)
Conjugal transfer of Bacteroides mobilizable transposon Tn4555 was examined with an Escherichia coli-based assay system. It was shown that mobilization required the cis-acting oriTTn region and that the Tn4555 mobATn gene ...
Cloning and Characterization of the Pseudomonas aeruginosa zwf Gene Encoding Glucose-6-Phosphate Dehydrogenase, an Enzyme Important in Resistance to Methyl Viologen (Paraquat)
(East Carolina University, 1998-04)
In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD1- or NADP1-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. ...
Characterization of a Peroxide-Resistant Mutant of the Anaerobic Bacterium Bacteroides fragilis
(East Carolina University, 1998-11)
A Bacteroides fragilis mutant resistant to hydrogen peroxide and alkyl peroxide was isolated by enrichment
in increasing concentrations of hydrogen peroxide. The mutant strain was constitutively resistant to 100 mM
H2O2 ...