Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans
Author
Fisher-Wellman, Kelsey H.; Weber, Todd M.; Cathey, Brook L.; Brophy, Patricia; Gilliam, Laura A. A.; Kane, Constance L.; Maples, Jill M.; Gavin, Timothy Patrick; Houmard, Joseph A.; Neufer, P. Darrell
Abstract
Considerable debate exists about whether alterations in mitochondrial respiratory capacity and/or content play a causal role in the development of insulin resistance during obesity. The current study was undertaken to determine whether such alterations are present during the initial stages of insulin resistance in humans. Young (∼23 years) insulin-sensitive lean and insulin-resistant obese men and women were studied. Insulin resistance was confirmed through an intravenous glucose tolerance test. Measures of mitochondrial respiratory capacity and content as well as H(2)O(2) emitting potential and the cellular redox environment were performed in permeabilized myofibers and primary myotubes prepared from vastus lateralis muscle biopsy specimens. No differences in mitochondrial respiratory function or content were observed between lean and obese subjects, despite elevations in H(2)O(2) emission rates and reductions in cellular glutathione. These findings were apparent in permeabilized myofibers as well as in primary myotubes. The results suggest that reductions in mitochondrial respiratory capacity and content are not required for the initial manifestation of peripheral insulin resistance.
Date
2014-01
Citation:
APA:
Fisher-Wellman, Kelsey H., & Weber, Todd M., & Cathey, Brook L., & Brophy, Patricia, & Gilliam, Laura A. A., & Kane, Constance L., & Maples, Jill M., & Gavin, Timothy Patrick, & Houmard, Joseph A., & Neufer, P. Darrell. (January 2014).
Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans.
Diabetes,
63(1),
132-
141. Retrieved from
http://hdl.handle.net/10342/5399
MLA:
Fisher-Wellman, Kelsey H., and Weber, Todd M., and Cathey, Brook L., and Brophy, Patricia, and Gilliam, Laura A. A., and Kane, Constance L., and Maples, Jill M., and Gavin, Timothy Patrick, and Houmard, Joseph A., and Neufer, P. Darrell.
"Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans". Diabetes.
63:1. (132-141),
January 2014.
December 11, 2023.
http://hdl.handle.net/10342/5399.
Chicago:
Fisher-Wellman, Kelsey H. and Weber, Todd M. and Cathey, Brook L. and Brophy, Patricia and Gilliam, Laura A. A. and Kane, Constance L. and Maples, Jill M. and Gavin, Timothy Patrick and Houmard, Joseph A. and Neufer, P. Darrell,
"Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans," Diabetes 63, no.
1 (January 2014),
http://hdl.handle.net/10342/5399 (accessed
December 11, 2023).
AMA:
Fisher-Wellman, Kelsey H., Weber, Todd M., Cathey, Brook L., Brophy, Patricia, Gilliam, Laura A. A., Kane, Constance L., Maples, Jill M., Gavin, Timothy Patrick, Houmard, Joseph A., Neufer, P. Darrell.
Mitochondrial Respiratory Capacity and Content Are Normal in Young Insulin-Resistant Obese Humans. Diabetes.
January 2014;
63(1):
132-141.
http://hdl.handle.net/10342/5399. Accessed
December 11, 2023.
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