Genome sequencing and analysis of the paclitaxel-producing endophytic fungus NRRL 62431
Yang, Yanfang; Zhao, Hainan; Barrero, Roberto A; Zhang, Baohong; Sun, Guiling; Wilson, Iain W.; Xie, Fuliang; Walker, Kevin D; Parks, Joshua W; Bruce, Robert; Guo, Guangwu; Chen, Li; Zhang, Yong; Huang, Xin; Tang, Qi; Liu, Hongwei; Bellgard, Matthew I; Qiu, Deyou; Lai, Jinsheng; Hoffman, Angela
Background Paclitaxel (Taxol™) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer. Results The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely. Conclusions Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi.
Yang, Yanfang, & Zhao, Hainan, & Barrero, Roberto A, & Zhang, Baohong, & Sun, Guiling, & Wilson, Iain W., & Xie, Fuliang, & Walker, Kevin D, & Parks, Joshua W, & Bruce, Robert, & Guo, Guangwu, & Chen, Li, & Zhang, Yong, & Huang, Xin, & Tang, Qi, & Liu, Hongwei, & Bellgard, Matthew I, & Qiu, Deyou, & Lai, Jinsheng, & Hoffman, Angela. (January 2014). Genome sequencing and analysis of the paclitaxel-producing endophytic fungus NRRL 62431. BMC Genomics, (69-69. Retrieved from http://hdl.handle.net/10342/5455
Yang, Yanfang, and Zhao, Hainan, and Barrero, Roberto A, and Zhang, Baohong, and Sun, Guiling, and Wilson, Iain W., and Xie, Fuliang, and Walker, Kevin D, and Parks, Joshua W, and Bruce, Robert, and Guo, Guangwu, and Chen, Li, and Zhang, Yong, and Huang, Xin, and Tang, Qi, and Liu, Hongwei, and Bellgard, Matthew I, and Qiu, Deyou, and Lai, Jinsheng, and Hoffman, Angela. "Genome sequencing and analysis of the paclitaxel-producing endophytic fungus NRRL 62431". BMC Genomics. . (69-69.), January 2014. July 12, 2020. http://hdl.handle.net/10342/5455.
Yang, Yanfang and Zhao, Hainan and Barrero, Roberto A and Zhang, Baohong and Sun, Guiling and Wilson, Iain W. and Xie, Fuliang and Walker, Kevin D and Parks, Joshua W and Bruce, Robert and Guo, Guangwu and Chen, Li and Zhang, Yong and Huang, Xin and Tang, Qi and Liu, Hongwei and Bellgard, Matthew I and Qiu, Deyou and Lai, Jinsheng and Hoffman, Angela, "Genome sequencing and analysis of the paclitaxel-producing endophytic fungus NRRL 62431," BMC Genomics 15, no. (January 2014), http://hdl.handle.net/10342/5455 (accessed July 12, 2020).
Yang, Yanfang, Zhao, Hainan, Barrero, Roberto A, Zhang, Baohong, Sun, Guiling, Wilson, Iain W., Xie, Fuliang, Walker, Kevin D, Parks, Joshua W, Bruce, Robert, Guo, Guangwu, Chen, Li, Zhang, Yong, Huang, Xin, Tang, Qi, Liu, Hongwei, Bellgard, Matthew I, Qiu, Deyou, Lai, Jinsheng, Hoffman, Angela. Genome sequencing and analysis of the paclitaxel-producing endophytic fungus NRRL 62431. BMC Genomics. January 2014; 15() 69-69. http://hdl.handle.net/10342/5455. Accessed July 12, 2020.