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    Differential gene expression and alternative splicing in insect immune specificity

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    Author
    Riddell, Carolyn E.; Lobaton Garces, Juan D.; Adams, Sally; Barribeau, Seth M.; Twell, David; Mallon, Eamonn B.
    Abstract
    Background Ecological studies routinely show genotype-genotype interactions between insects and their parasites. The mechanisms behind these interactions are not clearly understood. Using the bumblebee Bombus terrestris/trypanosome Crithidia bombi model system (two bumblebee colonies by two Crithidia strains), we have carried out a transcriptome-wide analysis of gene expression and alternative splicing in bees during C. bombi infection. We have performed four analyses, 1) comparing gene expression in infected and non-infected bees 24 hours after infection by Crithidia bombi, 2) comparing expression at 24 and 48 hours after C. bombi infection, 3) determining the differential gene expression associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours after infection and 4) determining the alternative splicing associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours post infection. Results We found a large number of genes differentially regulated related to numerous canonical immune pathways. These genes include receptors, signaling pathways and effectors. We discovered a possible interaction between the peritrophic membrane and the insect immune system in defense against Crithidia. Most interestingly, we found differential expression and alternative splicing of immunoglobulin related genes (Dscam and Twitchin) are associated with the genotype-genotype interactions of the given bumblebee colony and Crithidia strain. Conclusions In this paper we have shown that the expression and alternative splicing of immune genes is associated with specific interactions between different host and parasite genotypes in this bumblebee/trypanosome model.
    URI
    http://hdl.handle.net/10342/5492
    Subject
     Genotype-genotype; Peritrophic membrane; Social insects 
    Date
    2014-11
    Citation:
    APA:
    Riddell, Carolyn E., & Lobaton Garces, Juan D., & Adams, Sally, & Barribeau, Seth M., & Twell, David, & Mallon, Eamonn B.. (November 2014). Differential gene expression and alternative splicing in insect immune specificity. BMC Genomics, (15:1), p.1-15. Retrieved from http://hdl.handle.net/10342/5492

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    MLA:
    Riddell, Carolyn E., and Lobaton Garces, Juan D., and Adams, Sally, and Barribeau, Seth M., and Twell, David, and Mallon, Eamonn B.. "Differential gene expression and alternative splicing in insect immune specificity". BMC Genomics. 15:1. (1-15.), November 2014. April 19, 2021. http://hdl.handle.net/10342/5492.
    Chicago:
    Riddell, Carolyn E. and Lobaton Garces, Juan D. and Adams, Sally and Barribeau, Seth M. and Twell, David and Mallon, Eamonn B., "Differential gene expression and alternative splicing in insect immune specificity," BMC Genomics 15, no. 1 (November 2014), http://hdl.handle.net/10342/5492 (accessed April 19, 2021).
    AMA:
    Riddell, Carolyn E., Lobaton Garces, Juan D., Adams, Sally, Barribeau, Seth M., Twell, David, Mallon, Eamonn B.. Differential gene expression and alternative splicing in insect immune specificity. BMC Genomics. November 2014; 15(1) 1-15. http://hdl.handle.net/10342/5492. Accessed April 19, 2021.
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