Browsing Thomas Harriot College of Arts and Sciences by Author "Offenbacher, Adam R"
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Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases
Roberts, Daniella (East Carolina University, 2022-04-27)Lipoxygenases (LOXs) are a family of enzymes found in plants, animals, fungi, and bacteria that catalyze the per-oxidation of polyunsaturated fatty acids. In plants, LOXs are involved in growth, development, and defense ... -
Defining Protein Motions the Comprise the Reaction Barrier in Human Epithelial 15-Lipoxygenase-2
Ohler, Amanda (East Carolina University, 2022-04-27)Proteins are dynamic in nature, with these motions playing a role in substrate binding and product release. Protein thermal motions have emerged as participating in the bond making/breaking steps of catalysis and by extension ... -
DEVELOPMENT OF UNNATURAL SUBSTRATES AND TRYPTOPHAN AMINO ACIDS TO STUDY PROTON COUPLED ELECTRON TRANSFER IN ENZYMES
Ohgo, Kei (East Carolina University, 2020-12-09)Proton-coupled electron transfer plays an important role in substrate oxidation by C-H bond cleavage and long-range pathways associated with bioenergetics. This thesis is focused on the synthesis of unnatural substrates ... -
DIRECT ELECTROCHEMICAL ANALYSIS OF THE REDOX ACTIVITY OF TRYPTOPHAN AND TYROSINE IN MODIFIED AZURINS: THE IMPACT OF THE PROTEIN ENVIRONMENT
Tyson, Kris Janell (East Carolina University, 2021-05-03)Proton-coupled electron transfer (PCET) is a biological process essential to life. It is imperative for respiration in animals as well as photosynthesis in plants. Long-range PCET is often facilitated by redox-active amino ... -
THE IMPACT OF GLYCOSYLATION ON ACTIVE SITE STRUCTURE AND ACTIVITY IN THE FUNGUS ENZYME MOLOX
Kostenko, Anastasiia (East Carolina University, 2019-05-01)Lipoxygenases from pathogenic fungi belong to the lipoxygenase family that catalyze the C-H activation of polyunsaturated fatty acids to form diverse hydroperoxides. While the lipoxygenase catalytic domains are structurally ...