ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes
Author
Ding, Yong; Ndamukong, Ivan; Xu, Zaoshi; Lapko, Hanna; Fromm, Michael; Avramova, Zoya
Abstract
Tri-methylated H3 lysine 4 (H3K4me3) is associated with transcriptionally active genes, but its function in the transcription process is still unclear. Point mutations in the catalytic domain of ATX1 (ARABIDOPSIS TRITHORAX1), a H3K4 methyltransferase, and RNAi knockdowns of subunits of the AtCOMPASS–like (Arabidopsis Complex Proteins Associated with Set) were used to address this question. We demonstrate that both ATX1 and AtCOMPASS–like are required for high level accumulation of TBP (TATA-binding protein) and Pol II at promoters and that this requirement is independent of the catalytic histone modifying activity. However, the catalytic function is critically required for transcription as H3K4me3 levels determine the efficiency of transcription elongation. The roles of H3K4me3, ATX1, and AtCOMPASS–like may be of a general relevance for transcription of Trithorax-activated eukaryotic genes.
Date
2012-12
Citation:
APA:
Ding, Yong, & Ndamukong, Ivan, & Xu, Zaoshi, & Lapko, Hanna, & Fromm, Michael, & Avramova, Zoya. (December 2012).
ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes.
PLoS Genetics,
8(12),
1-
12. Retrieved from
http://hdl.handle.net/10342/5657
MLA:
Ding, Yong, and Ndamukong, Ivan, and Xu, Zaoshi, and Lapko, Hanna, and Fromm, Michael, and Avramova, Zoya.
"ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes". PLoS Genetics.
8:12. (1-12),
December 2012.
December 10, 2023.
http://hdl.handle.net/10342/5657.
Chicago:
Ding, Yong and Ndamukong, Ivan and Xu, Zaoshi and Lapko, Hanna and Fromm, Michael and Avramova, Zoya,
"ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes," PLoS Genetics 8, no.
12 (December 2012),
http://hdl.handle.net/10342/5657 (accessed
December 10, 2023).
AMA:
Ding, Yong, Ndamukong, Ivan, Xu, Zaoshi, Lapko, Hanna, Fromm, Michael, Avramova, Zoya.
ATX1-Generated H3K4me3 Is Required for Efficient Elongation of Transcription, Not Initiation, at ATX1-Regulated Genes. PLoS Genetics.
December 2012;
8(12):
1-12.
http://hdl.handle.net/10342/5657. Accessed
December 10, 2023.
Collections