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    Regulation of the Mitogen Activated Protein Kinase Kinase (MEK)-1 by NAD-Dependent Deacetylases

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    Author
    Yeung, Fan; Ramsey, Catherine S.; Popko-Scibor, Anita E.; Allison, David F.; Gray, Lisa G.; Shin, Min; Kumar, Manish; Li, Duo; McCubrey, James A.; Mayo, Marty W.
    Abstract
    Sirtuins are class III deacetylases that regulate many essential processes, including cellular stress, genome stability, and metabolism. Although these NAD+-dependent deacetylases control adaptive cellular responses, identification of sirtuin-regulated signaling targets remain under-studied. Here, we demonstrate that acetylation of the mitogen-activated protein kinase kinase-1 (MEK1) stimulates its kinase activity, and that acetylated MEK1 is under the regulatory control of the sirtuin family members SIRT1 and SIRT2. Treatment of cells with sirtuin inhibitors, or siRNA knockdown of SIRT1 or SIRT2 proteins, increases MEK1 acetylation and subsequent phosphorylation of the extracellular signal-regulated kinase (ERK). Generation of an acetyl-specific MEK1 antibody demonstrates that endogenous acetylated MEK1 is extensively enriched in the nucleus following epidermal growth factor (EGF) stimulation. An acetyl-mimic of MEK1 increases inappropriate growth properties, suggesting that acetylation of MEK1 has oncogenic potential.
    URI
    http://hdl.handle.net/10342/5675
    Subject
     MEK1; sirtuins; acetylation; growth promoting; oncogenic 
    Date
    2014-02
    Citation:
    APA:
    Yeung, Fan, & Ramsey, Catherine S., & Popko-Scibor, Anita E., & Allison, David F., & Gray, Lisa G., & Shin, Min, & Kumar, Manish, & Li, Duo, & McCubrey, James A., & Mayo, Marty W.. (February 2014). Regulation of the Mitogen Activated Protein Kinase Kinase (MEK)-1 by NAD-Dependent Deacetylases. Oncogene, 34(6), 798- 804. Retrieved from http://hdl.handle.net/10342/5675

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    MLA:
    Yeung, Fan, and Ramsey, Catherine S., and Popko-Scibor, Anita E., and Allison, David F., and Gray, Lisa G., and Shin, Min, and Kumar, Manish, and Li, Duo, and McCubrey, James A., and Mayo, Marty W.. "Regulation of the Mitogen Activated Protein Kinase Kinase (MEK)-1 by NAD-Dependent Deacetylases". Oncogene. 34:6. (798-804), February 2014. September 25, 2023. http://hdl.handle.net/10342/5675.
    Chicago:
    Yeung, Fan and Ramsey, Catherine S. and Popko-Scibor, Anita E. and Allison, David F. and Gray, Lisa G. and Shin, Min and Kumar, Manish and Li, Duo and McCubrey, James A. and Mayo, Marty W., "Regulation of the Mitogen Activated Protein Kinase Kinase (MEK)-1 by NAD-Dependent Deacetylases," Oncogene 34, no. 6 (February 2014), http://hdl.handle.net/10342/5675 (accessed September 25, 2023).
    AMA:
    Yeung, Fan, Ramsey, Catherine S., Popko-Scibor, Anita E., Allison, David F., Gray, Lisa G., Shin, Min, Kumar, Manish, Li, Duo, McCubrey, James A., Mayo, Marty W.. Regulation of the Mitogen Activated Protein Kinase Kinase (MEK)-1 by NAD-Dependent Deacetylases. Oncogene. February 2014; 34(6): 798-804. http://hdl.handle.net/10342/5675. Accessed September 25, 2023.
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