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    Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation of Neural Stem Cells Mainly through PI3-K/Akt Pathways

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    Author
    Cai, Ming; Zhou, Yuehui; Zhou, Bin; Lou, Shujie
    Abstract
    Purpose To investigate the effects of hypoxic conditioned media from rat cerebral cortical cells on the proliferation and differentiation of neural stem cells (NSCs) in vitro, and to study the roles of PI3-K/Akt and JNK signal transduction pathways in these processes. Methods Cerebral cortical cells from neonatal Sprague–Dawley rat were cultured under hypoxic and normoxic conditions; the supernatant was collected and named ‘hypoxic conditioned medium’ (HCM) and ‘normoxic conditioned medium’ (NCM), respectively. We detected the protein levels (by ELISA) of VEGF and BDNF in the conditioned media and mRNA levels (by RT-PCR) in cerebral cortical cells. The proliferation (number and size of neurospheres) and differentiation (proportion of neurons and astrocytes over total cells) of NSCs was assessed. LY294002 and SP600125, inhibitors of PI3-K/Akt and JNK, respectively, were applied, and the phosphorylation levels of PI3-K, Akt and JNK were measured by western blot. Results The protein levels and mRNA expressions of VEGF and BDNF in 4% HCM and 1% HCM were both higher than that of those in NCM. The efficiency and speed of NSCs proliferation was enhanced in 4% HCM compared with 1% HCM. The highest percentage of neurons and lowest percentage of astrocytes was found in 4% HCM. However, the enhancement of NSCs proliferation and differentiation into neurons accelerated by 4% HCM was inhibited by LY294002 and SP600125, with LY294002 having a stronger inhibitory effect. The increased phosphorylation levels of PI3-K, Akt and JNK in 4% HCM were blocked by LY294002 and SP600125. Conclusions 4%HCM could promote NSCs proliferation and differentiation into high percentage of neurons, these processes may be mainly through PI3-K/Akt pathways.
    URI
    http://hdl.handle.net/10342/5778
    Subject
     Neurons; Neurospheres; Astrocytes; Cell differentiation; Nuclear staining 
    Date
    2014
    Citation:
    APA:
    Cai, Ming, & Zhou, Yuehui, & Zhou, Bin, & Lou, Shujie. (January 2014). Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation of Neural Stem Cells Mainly through PI3-K/Akt Pathways. PLoS ONE, (9:11), p.1-10. Retrieved from http://hdl.handle.net/10342/5778

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Cai, Ming, and Zhou, Yuehui, and Zhou, Bin, and Lou, Shujie. "Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation of Neural Stem Cells Mainly through PI3-K/Akt Pathways". PLoS ONE. 9:11. (1-10.), January 2014. April 20, 2021. http://hdl.handle.net/10342/5778.
    Chicago:
    Cai, Ming and Zhou, Yuehui and Zhou, Bin and Lou, Shujie, "Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation of Neural Stem Cells Mainly through PI3-K/Akt Pathways," PLoS ONE 9, no. 11 (January 2014), http://hdl.handle.net/10342/5778 (accessed April 20, 2021).
    AMA:
    Cai, Ming, Zhou, Yuehui, Zhou, Bin, Lou, Shujie. Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation of Neural Stem Cells Mainly through PI3-K/Akt Pathways. PLoS ONE. January 2014; 9(11) 1-10. http://hdl.handle.net/10342/5778. Accessed April 20, 2021.
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