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Now showing items 31-40 of 70
Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers
(East Carolina University, 2018-07-24)
It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) ...
protaTETHER – a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc‐GFP fusion protein
(2018-03-14)
Protein fusions are of fundamental importance in the study of cellular biology and the elucidation of cell signaling pathways, and the importance of linkers for the proper function of protein fusions is well documented in ...
Thermodynamic and spectroscopic studies of Cd²⁺ binding to the regulatory domain and full length human cardiac troponin C (HcTnC) : elucidating plausible Cd²⁺ binding sites
(East Carolina University, 2015)
Toxic metals such as cadmium (Cd²⁺) have been shown to bind to and interfere with various calcium (Ca²⁺) binding proteins including the regulatory protein cardiac troponin C (cTnC). Recent structural data has shown that ...
Investigation Into The Scope, Limitations, And Mechanistic Aspects Of A Regioselective Acylation Of Cycloplatinated Complexes
(East Carolina University, 2015)
In the reaction of N-isopropyl-N-phenyl-2,2'-bipyridin-6-amine with potassium tetrachloroplatinate, the selective C-H bond activation was complicated by the low selectivity of sp² C-H bond activation in acetonitrile and ...
Synthesis Of Non-Natural Fmoc-Protected Amino Acids To Provide Novel Fluorescent Anion Probes And Their Incorporation Into Synthetic Peptides
(East Carolina University, 2015)
Cystic fibrosis is a genetic disorder caused by mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. CFTR serves to control the gradient of chloride and bicarbonate ions across the cellular ...
Method Development and Validation for the Rapid Detection of Organophosphates in Blood Plasma from Obese, Obese-Diabetic, and Lean Patients from Eastern North Carolina
(East Carolina University, 2017-07-13)
The incidence of diabetes and rate of obesity is on the rise, along with the use of organophosphorus insecticides in the United States. Organophosphates (OP), a specific class of pesticide that is biodegradable and readily ...
SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF BOC- PROTECTED FLUORESCENT ARGININE
(East Carolina University, 2017-08-21)
Small peptides capable of penetrating or spanning the hydrophobic core of lipid bilayers have many potential uses in medicine and in biochemical research. As the most basic of the amino acids that can comprise such peptides, ...
ANALYSIS OF OXYGENATED POLYCYCLIC AROMATIC HYDROCARBONS AND THEIR PARENT MOLECULES IN BIOCHAR AND ITS LEACHATES
(East Carolina University, 2017-12-13)
Due to the increasing use of biochar as a soil amendment, the amount and composition of contaminants in biochar should be analyzed. Two of those suites of compounds in biochar are polycyclic aromatic hydrocarbons (PAHs) ...
Fluorescent Derivatives of Prostamides: Tools for Studying Anti-Cancer Activity
(East Carolina University, 2018-07-23)
The endocannabinoid arachidonoyl ethanolamide (AEA) and prostaglandins derived from it selectively induce apoptosis in tumorigenic cell lines due to overexpression of COX-2. It is likely that a novel J-series prostamide, ...
THE DEVELOPMENT OF BETA-ACTIN MUTANTS WITH ALTERED BINDING AFFINITES FOR ATP AND ADP
(East Carolina University, 2018-12-10)
A number of neurodegenerative diseases are accompanied by the formation of cofilin-actin rods in neural cells. These rods can cause synaptic dysfunction and block transport within neurites. The overall goal of this project ...