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Fundamental and Applied Studies on Chromatographic Separation of Cold Drugs and Skincare Creams and Extraction of Salvia miltiorrhiza using Subcritical Water
(East Carolina University, 2014)
Subcritical water chromatography (SBWC) and subcritical water extraction (SBWE) are two green techniques that use subcritical water as the sole solvent for separations, thus eliminating the use of toxic and expensive organic ...
Investigation of the Zinc binding region of Prothymosin-alpha : A spectroscopic and thermodynamic approach to study metal binding in Intrinsically disordered proteins
(East Carolina University, 2014)
The goal of this study is to provide a deeper insight into how metals bind intrinsically disordered proteins (IDPs) by taking zinc binding to prothymosin-alpha as a case study. The involvement of metals in several diseases ...
Defining the Role of the C-Terminal Region of Troponin T by Analysis of a Series of Truncation Mutants
(East Carolina University, 2016-07-21)
Familial hypertrophic cardiomyopathy and other cardiovascular diseases result from mutations of any of the contractile proteins. Mutations within the actin binding regulatory complex of proteins, including tropomyosin (Tm) ...
THE SYNTHESIS, STRUCTURE AND PHOTOPHYSICAL PROPERTIES OF PHOSPHORESCENT CYCLOMETALATED N^N^C-COORDINATED PLATINUM COMPLEXES
(East Carolina University, 2016-07-25)
Phosphorescent materials based on cyclometalated platinum complexes have attracted a great deal of attention because of their potential in chemical, biological and optoelectronic applications, specifically as emitters in ...
Electrochemical Detection of Benzo[a]Pyrene-Induced DNA Damage at TP53 Oligomers : Impact of 5'-Methyl Cytosine and Bioactivation on the Genotoxicity Process
(East Carolina University, 2014)
DNA houses the blueprint that dictates how an organism will develop. However, DNA features numerous reactive sites that can be attacked by chemicals and radiation, resulting in DNA damage and possibly mutations. Chemical ...
protaTETHER – a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc‐GFP fusion protein
(2018-03-14)
Protein fusions are of fundamental importance in the study of cellular biology and the elucidation of cell signaling pathways, and the importance of linkers for the proper function of protein fusions is well documented in ...
Heavy Metal Concentrations in Biofluid Samples and their Correlation to Metabolic Syndrome
(East Carolina University, 2018-05-01)
The goal of this project was to determine if toxic metals such as Cr, Mn, As, Ni, Cd, and Pb are stored in the adipose tissue of farm animals, in particular swine. To detect these trace elements, a method of extraction and ...
Membrane Localization of Pyrene-Trehalose Conjugates (PYRETs)
(East Carolina University, 2018-05-01)
The disaccharide trehalose is found in many organisms that can survive in extremely cold or dry environments. According to the “hydration hypothesis,” trehalose interacts with the glycerol/phosphate regions of lipid bilayers ...
Using LC/MS to Quantify Metabolites in Urine Samples Post Clinical Exposure to Benzoates in Beverages
(East Carolina University, 2018-05-01)
Evidence has suggested that benzoate consumption has the potential to cause weight loss prevention. Benzoate is conjugated with glycine in the mitochondrial matrix to form hippurate which is eliminated in urine. IRB approval ...
Detection of Environmental DNA Damage Using Electrochemical Sensors and Mass Spectrometry
(East Carolina University, 2018-05-04)
Nickel exposure has been linked to a variety of health complications including instances of tumors and cancer in living organisms. Exposure to nickel can occur due to pollution from mining or industrial applications. ...