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THE IMPACT OF GLYCOSYLATION ON ACTIVE SITE STRUCTURE AND ACTIVITY IN THE FUNGUS ENZYME MOLOX
(East Carolina University, 2019-05-01)
Lipoxygenases from pathogenic fungi belong to the lipoxygenase family that catalyze the C-H activation of polyunsaturated fatty acids to form diverse hydroperoxides. While the lipoxygenase catalytic domains are structurally ...
Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases
(East Carolina University, 2022-04-27)
Lipoxygenases (LOXs) are a family of enzymes found in plants, animals, fungi, and bacteria that catalyze the per-oxidation of polyunsaturated fatty acids. In plants, LOXs are involved in growth, development, and defense ...
Defining Protein Motions the Comprise the Reaction Barrier in Human Epithelial 15-Lipoxygenase-2
(East Carolina University, 2022-04-27)
Proteins are dynamic in nature, with these motions playing a role in substrate binding and product release. Protein thermal motions have emerged as participating in the bond making/breaking steps of catalysis and by extension ...
DEVELOPMENT OF UNNATURAL SUBSTRATES AND TRYPTOPHAN AMINO ACIDS TO STUDY PROTON COUPLED ELECTRON TRANSFER IN ENZYMES
(East Carolina University, 2020-12-09)
Proton-coupled electron transfer plays an important role in substrate oxidation by C-H bond cleavage and long-range pathways associated with bioenergetics. This thesis is focused on the synthesis of unnatural substrates ...