Chemistry: Recent submissions
Now showing items 21-40 of 101
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Elucidating the Mechanisms of Transthyretin Aggregation in Transthyretin Amyloidosis
(East Carolina University, 2018-07-24)Accumulation of insoluble aggregates (amyloids) is a characteristic feature of many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. Aggregation and deposition of Transthyretin (TTR) protein ... -
Counting Protons: Using Isothermal Titration Calorimetry to Determine the Number of Protons Exchanged Upon Ca(II) and Cd(II) Binding to Wild-type HcTnC and Mutant C84A and C35A/C84A Proteins
(East Carolina University, 2018-07-20)Maintaining a delicate balance of metal ions in the cell is crucial to the survival of many organisms. Divalent cadmium, Cd(II), is a non-essential metal ion that can disrupt this delicate balance and lead to deleterious ... -
Fluorescent Derivatives of Prostamides: Tools for Studying Anti-Cancer Activity
(East Carolina University, 2018-07-23)The endocannabinoid arachidonoyl ethanolamide (AEA) and prostaglandins derived from it selectively induce apoptosis in tumorigenic cell lines due to overexpression of COX-2. It is likely that a novel J-series prostamide, ... -
Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers
(East Carolina University, 2018-07-24)It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) ... -
Using LC/MS to Quantify Metabolites in Urine Samples Post Clinical Exposure to Benzoates in Beverages
(East Carolina University, 2018-05-01)Evidence has suggested that benzoate consumption has the potential to cause weight loss prevention. Benzoate is conjugated with glycine in the mitochondrial matrix to form hippurate which is eliminated in urine. IRB approval ... -
Membrane Localization of Pyrene-Trehalose Conjugates (PYRETs)
(East Carolina University, 2018-05-01)The disaccharide trehalose is found in many organisms that can survive in extremely cold or dry environments. According to the “hydration hypothesis,” trehalose interacts with the glycerol/phosphate regions of lipid bilayers ... -
Heavy Metal Concentrations in Biofluid Samples and their Correlation to Metabolic Syndrome
(East Carolina University, 2018-05-01)The goal of this project was to determine if toxic metals such as Cr, Mn, As, Ni, Cd, and Pb are stored in the adipose tissue of farm animals, in particular swine. To detect these trace elements, a method of extraction and ... -
protaTETHER – a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc‐GFP fusion protein
(2018-03-14)Protein fusions are of fundamental importance in the study of cellular biology and the elucidation of cell signaling pathways, and the importance of linkers for the proper function of protein fusions is well documented in ... -
Detection of Environmental DNA Damage Using Electrochemical Sensors and Mass Spectrometry
(East Carolina University, 2018-05-04)Nickel exposure has been linked to a variety of health complications including instances of tumors and cancer in living organisms. Exposure to nickel can occur due to pollution from mining or industrial applications. ... -
ANALYSIS OF OXYGENATED POLYCYCLIC AROMATIC HYDROCARBONS AND THEIR PARENT MOLECULES IN BIOCHAR AND ITS LEACHATES
(East Carolina University, 2017-12-13)Due to the increasing use of biochar as a soil amendment, the amount and composition of contaminants in biochar should be analyzed. Two of those suites of compounds in biochar are polycyclic aromatic hydrocarbons (PAHs) ... -
SYNTHESIS AND SPECTROSCOPIC PROPERTIES OF BOC- PROTECTED FLUORESCENT ARGININE
(East Carolina University, 2017-08-21)Small peptides capable of penetrating or spanning the hydrophobic core of lipid bilayers have many potential uses in medicine and in biochemical research. As the most basic of the amino acids that can comprise such peptides, ... -
Method Development and Validation for the Rapid Detection of Organophosphates in Blood Plasma from Obese, Obese-Diabetic, and Lean Patients from Eastern North Carolina
(East Carolina University, 2017-07-13)The incidence of diabetes and rate of obesity is on the rise, along with the use of organophosphorus insecticides in the United States. Organophosphates (OP), a specific class of pesticide that is biodegradable and readily ... -
The Thermodynamics of Cd(II) Binding to Human Cardiac Troponin C: Using Mutants to Determine the Location of Cd(II) Binding
(East Carolina University, 2017-05-31)Historically, cadmium (Cd) has been used in many different applications from cadmium paint to nickel-cadmium batteries. However, Cd is a nonessential, naturally occurring toxic element due to its low excretion rate and ... -
Platinum-Catalyzed Acylation of 2-(aryloxy)pyridines Via Direct Carbon-Hydrogen Activation
(East Carolina University, 2017-05-24)Carbonyl groups are ubiquitous in pharmaceuticals, biomolecules, and natural products. The traditional means of acylation to introduce carbonyls, such as the Friedel-Crafts acylation, can be limited in scope. More recent ... -
DIFFERENTIATION OF J-SERIES PROSTAGLANDINS AS PRO-APOPTOTIC PRODUCTS OF CANCER CELL METABOLISM
(East Carolina University, 2016-12-16)Cyclopentenone J-series prostaglandins and prostaglandin-ethanolamide conjugates have been shown to selectively induce endoplasmic reticulum stress-apoptosis in different cancer cell types. These lipids have been identified ... -
Investigation of possible driving forces on counter-intuitive thermal collapse of intrinsically disordered proteins
(East Carolina University, 2016-08-18)Intrinsically disordered proteins are an emerging class of proteins that contain no secondary or tertiary structure. Through bioinformatics studies it has been predicted that a third of the human proteome consists of ... -
Defining the Role of the C-Terminal Region of Troponin T by Analysis of a Series of Truncation Mutants
(East Carolina University, 2016-07-21)Familial hypertrophic cardiomyopathy and other cardiovascular diseases result from mutations of any of the contractile proteins. Mutations within the actin binding regulatory complex of proteins, including tropomyosin (Tm) ... -
THE SYNTHESIS, STRUCTURE AND PHOTOPHYSICAL PROPERTIES OF PHOSPHORESCENT CYCLOMETALATED N^N^C-COORDINATED PLATINUM COMPLEXES
(East Carolina University, 2016-07-25)Phosphorescent materials based on cyclometalated platinum complexes have attracted a great deal of attention because of their potential in chemical, biological and optoelectronic applications, specifically as emitters in ... -
Utilizing Isothermal Titration Calorimetry to Quantify the Thermodynamics of Cadmium Mimicry: A Comparison to Calcium
(East Carolina University, 2016-07-20)Isothermal titration calorimetry (ITC) is a powerful calorimetric method which can determine all thermodynamic parameters (K, [Delta]H, [Delta]G, and [Delta]S) for a binding event in a single experiment. More recently, ITC ... -
Using Fluorescent Probes to Study the Location and Interaction of Disaccharides within Lipid Bilayers
(East Carolina University, 2016-04-26)It is well known that certain organisms, such as the tardigrade, are capable of surviving extreme conditions including dehydration, freezing and / or oxygen deficiency. The tardigrades ability to withstand such extreme ...