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    A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis

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    Author
    Ables, Elizabeth Tweedie; Hwang, Grace H.; Finger, Danielle S.; Hinnant, Taylor D.; Drummond-Barbosa, Daniela
    Abstract
    Multiple aspects of Drosophila oogenesis, including germline stem cell activity, germ cell differentiation, and follicle survival, are regulated by the steroid hormone ecdysone. While the transcriptional targets of ecdysone signaling during development have been studied extensively, targets in the ovary remain largely unknown. Early studies of salivary gland polytene chromosomes led to a model in which ecdysone stimulates a hierarchical transcriptional cascade, wherein a core group of ecdysone-sensitive transcription factors induce tissue-specific responses by activating secondary branches of transcriptional targets. More recently, genome-wide approaches have identified hundreds of putative ecdysone-responsive targets. Determining whether these putative targets represent bona fide targets in vivo, however, requires that they be tested via traditional mutant analysis in a cell-type specific fashion. To investigate the molecular mechanisms whereby ecdysone signaling regulates oogenesis, we used genetic mosaic analysis to screen putative ecdysone-responsive genes for novel roles in the control of the earliest steps of oogenesis. We identified a cohort of genes required for stem cell maintenance, stem and progenitor cell proliferation, and follicle encapsulation, growth, and survival. These genes encode transcription factors, chromatin modulators, and factors required for RNA transport, stability, and ribosome biogenesis, suggesting that ecdysone might control a wide range of molecular processes during oogenesis. Our results suggest that, although ecdysone target genes are known to have cell type-specific roles, many ecdysone response genes that control larval or pupal cell types at developmental transitions are used reiteratively in the adult ovary. These results provide novel insights into the molecular mechanisms by which ecdysone signaling controls oogenesis, laying new ground for future studies.
    Description
    Information about the supplemental materials can be found here: www.g3journal.org/lookup/suppl/doi:10.1534/g3.116.028951/-/DC1
    URI
    http://hdl.handle.net/10342/5869
    Subject
     Follicle cells; Germline; Nuclear hormone receptor; Stem cells; Steroid hormone 
    Date
    2016-08
    Citation:
    APA:
    Ables, Elizabeth Tweedie, & Hwang, Grace H., & Finger, Danielle S., & Hinnant, Taylor D., & Drummond-Barbosa, Daniela. (August 2016). A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis. , (), - . Retrieved from http://hdl.handle.net/10342/5869

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Ables, Elizabeth Tweedie, and Hwang, Grace H., and Finger, Danielle S., and Hinnant, Taylor D., and Drummond-Barbosa, Daniela. "A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis". . . (), August 2016. September 30, 2023. http://hdl.handle.net/10342/5869.
    Chicago:
    Ables, Elizabeth Tweedie and Hwang, Grace H. and Finger, Danielle S. and Hinnant, Taylor D. and Drummond-Barbosa, Daniela, "A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis," , no. (August 2016), http://hdl.handle.net/10342/5869 (accessed September 30, 2023).
    AMA:
    Ables, Elizabeth Tweedie, Hwang, Grace H., Finger, Danielle S., Hinnant, Taylor D., Drummond-Barbosa, Daniela. A Genetic Mosaic Screen Reveals Ecdysone-Responsive Genes Regulating Drosophila Oogenesis. . August 2016; (): . http://hdl.handle.net/10342/5869. Accessed September 30, 2023.
    Collections
    • 2016-2017 Open Access Publishing Fund
    • Biology
    • Open Access Fund
    • Open Access Publishing Support Fund

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