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    Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes

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    Author
    Fisher-Wellman, Kelsey H.; Davidson, Michael T.; Narowski, Tara M.; Lin, Chien-Te; Koves, Timothy R.; Muoio, Deborah M.
    Abstract
    Chronic metabolic diseases have been linked to molecular signatures of mitochondrial dysfunction. Nonetheless, molecular remodeling of the transcriptome, proteome, and/or metabolome does not necessarily translate to functional consequences that confer physiologic phenotypes. The work here aims to bridge the gap between molecular and functional phenomics by developing and validating a multiplexed assay platform for comprehensive assessment of mitochondrial energy transduction. The diagnostic power of the platform stems from a modified version of the creatine kinase energetic clamp technique, performed in parallel with multiplexed analyses of dehydrogenase activities and ATP synthesis rates. Together, these assays provide diagnostic coverage of the mitochondrial network at a level approaching that gained by molecular “-omics” technologies. Application of the platform to a comparison of skeletal muscle versus heart mitochondria reveals mechanistic insights into tissue-specific distinctions in energy transfer efficiency. This platform opens exciting opportunities to unravel the connection between mitochondrial bioenergetics and human disease.
    URI
    http://hdl.handle.net/10342/7680
    Date
    2018-09-25
    Citation:
    APA:
    Fisher-Wellman, Kelsey H., & Davidson, Michael T., & Narowski, Tara M., & Lin, Chien-Te, & Koves, Timothy R., & Muoio, Deborah M.. (September 2018). Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. , (), - . Retrieved from http://hdl.handle.net/10342/7680

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Fisher-Wellman, Kelsey H., and Davidson, Michael T., and Narowski, Tara M., and Lin, Chien-Te, and Koves, Timothy R., and Muoio, Deborah M.. "Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes". . . (), September 2018. October 01, 2023. http://hdl.handle.net/10342/7680.
    Chicago:
    Fisher-Wellman, Kelsey H. and Davidson, Michael T. and Narowski, Tara M. and Lin, Chien-Te and Koves, Timothy R. and Muoio, Deborah M., "Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes," , no. (September 2018), http://hdl.handle.net/10342/7680 (accessed October 01, 2023).
    AMA:
    Fisher-Wellman, Kelsey H., Davidson, Michael T., Narowski, Tara M., Lin, Chien-Te, Koves, Timothy R., Muoio, Deborah M.. Mitochondrial Diagnostics: A Multiplexed Assay Platform for Comprehensive Assessment of Mitochondrial Energy Fluxes. . September 2018; (): . http://hdl.handle.net/10342/7680. Accessed October 01, 2023.
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